Mutations that increase the activity of a transcriptional activator in yeast and mammalian cells.
Mutations that increase the activity of a transcriptional activator in yeast and mammalian cells.
复制标题
增加酵母和哺乳动物细胞中转录激活剂活性的突变。
DOI：
10.1073/pnas.87.6.2127
复制
发表时间：
1990
影响因子：
11.1
通讯作者：
Ptashne,M
中科院分区：
文献类型：
--
作者：
Gill,G;Sadowski,I;Ptashne,M
GAL4蛋白的激活区I是一段氨基酸，位于DNA结合区附近，激活酵母和哺乳动物细胞中的转录。在这里，我们描述了位于整个65个氨基酸区域的突变，增加了区域I的激活功能。这些突变中的大多数用中性氨基酸替换该区域中带正电荷的氨基酸，尽管我们也描述了在一个位置不改变该区域电荷的取代。当在哺乳动物细胞中测定时，在酵母中改变激活功能的区域I的突变对激活具有类似的影响。当单独的突变，提高了激活区的酸性重组，活性的突变蛋白质增加，增加负电荷在酵母和哺乳动物细胞。这些结果扩展和修改酸度和激活之间的相关性，并建议在酵母和哺乳动物中保守的要求，一个强大的激活区域。
Activating region I of GAL4 protein is a stretch of amino acids, positioned adjacent to the DNA-binding region, that activates transcription in yeast and, as we show here, in mammalian cells. Here we describe mutations located throughout a 65-amino acid region that increase the activation function of region I. Most of these mutations replace positively charged amino acids in the region with neutral ones, although we also describe substitutions at one position that do not alter the charge of the region. Mutations of region I that alter the activation function in yeast have similar effects on activation when assayed in mammalian cells. When individual mutations that raise the acidity of the activating region are recombined, the activities of the mutant proteins increase with increasing negative charge in both yeast and mammalian cells. These results extend and modify the correlation between acidity and activation and suggest that the requirements for a strong activating region are conserved in yeast and mammals.