In vitro biological evaluation of high molecular weight hyperbranched polyglycerols
In vitro biological evaluation of high molecular weight hyperbranched polyglycerols
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DOI：
10.1016/j.biomaterials.2007.07.011
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发表时间：
2007-11-01
期刊：
影响因子：
14
通讯作者：
Brooks, Donald Elliott
中科院分区：
文献类型：
--
作者：
Kainthan, Rajesh Kumar;Hester, Samuel R.;Brooks, Donald Elliott
低相对分子质量超支化聚甘油是一种具有高度水溶性和生物相容性的聚醚多元醇，可通过可控方式合成，具有窄的多分散性。最近，我们报道了超高相对分子质量(M-n高达70万)和窄分散的聚甘油的合成和表征，这些聚甘油有可能作为难以制备的高世代树枝状大分子的替代品。在体外对这些聚合物进行了详细的生物相容性测试。体外研究包括对凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、血浆再钙时间(PRT)、血栓弹力图参数(TEG)、补体激活、血小板激活、红细胞聚集和细胞毒性的影响的血液相容性测试。这些研究结果表明，这些高相对分子质量的聚甘油具有高度的生物相容性，在纳米生物技术和纳米医学中具有潜在的应用前景。此外，这些聚合物在热和氧化方面都是稳定的。(C)2007爱思唯尔有限公司。保留所有权利。
Low molecular weight hyperbranched polyglycerols are highly water soluble and biocompatible polyether polyols, which can be synthesized in a controlled manner with narrow polydispersity. Recently we reported the synthesis and characterization of very high molecular weight (M-n up to 700,000) and narrowly polydispersed polyglycerols which could be potentially used as alternatives to high generation dendrimers which are difficult to make. A detailed biocompatibility testing of these polymers conducted in vitro is reported here. The in vitro studies include hemocompatibility testing for effects on coagulation (prothrombin time (PT), activated partial thromboplastin time (APTT), plasma recalcification time (PRT), thrombelastograph parameters (TEG)), complement activation, platelet activation, red blood cell aggregation and cytotoxicity. Results from these studies show that these high molecular weight polyglycerols are highly biocompatible and are potential candidates for various applications in nanobiotechnology and in nanomedicine. Moreover these polymers are thermally and oxidatively stable. (c) 2007 Elsevier Ltd. All rights reserved.