Fine mapping and candidate gene analysis of qHD1b, a QTL that promotes flowering in common wild rice (Oryza rufipogon) by up-regulating Ehd1
Fine mapping and candidate gene analysis of qHD1b, a QTL that promotes flowering in common wild rice (Oryza rufipogon) by up-regulating Ehd1
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qHD1b 的精细定位和候选基因分析，qHD1b 是通过上调 Ehd1 促进普通野生稻 (Oryza rufipogon) 开花的 QTL
DOI：
10.1016/j.cj.2021.12.009
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发表时间：
2022-08-01
期刊：
影响因子：
6.6
通讯作者：
Wu, Weixun
中科院分区：
文献类型：
--
作者：
Liu, Ling;Zhang, Yingxin;Wu, Weixun
抽穗期（开花时间）决定了品种对不同环境的适应性。我们报告了 qHD1b 的精细定位和候选基因分析，qHD1b 是一个负责早期开花的数量性状位点（QTL），源自普通野生稻（O. rufipogon），在短日照和长日照条件下均如此。基因渗入系 IL7391 携带中汇 8015 (ZH8015) 背景中普通野生稻的片段，与背景相比表现出早抽穗，并与 ZH8015 杂交产生用于 QTL 分析的 BC5F2:3 家族。这使得能够鉴定两个抽穗期QTL，命名为qHD1b和qHD7，其中第一个被选择用于进一步研究。在BC5F4:5和BC5F6群体中进行高分辨率连锁分析，qHD1b的位置被限制在包含17个预测基因的112.7-kb区间内。其中五个基因在启动子或编码区含有多态性，因此被视为候选基因。表达分析显示 LOC_Os01g11940 表达与早期抽穗之间呈正相关。该基因座被注释为 OsFTL1，它编码拟南芥开花基因座 T 的直系同源基因，并且是 qHD1b 最有可能的候选基因。我们的研究表明，qHD1b 作为花激活剂，在短日照和长日照条件下通过上调 Ehd1、Hd3a、RFT1、OsMADS14 和 OsMADS15 来促进开花。田间试验表明，qHD1b 影响多个与产量相关的农艺性状，包括千粒重和粒长。 qHD1b 可用于早熟品种的标记辅助选择和育种。 (C) 2022 中国作物学会、中国农业科学院作物科学研究所。由Elsevier B.V. 代表科爱通讯有限公司制作和主持
Heading date (flowering time) determines the adaptability of cultivars to different environments. We report the fine mapping and candidate gene analysis of qHD1b, a quantitative trait locus (QTL) responsible for early flowering that was derived from common wild rice (O. rufipogon) under both short-day and long-day conditions. The introgression line IL7391, which carried segments from common wild rice in a Zhonghui 8015 (ZH8015) background, exhibited early heading compared to the background and was crossed with ZH8015 to generate BC5F2:3 families for QTL analysis. This enabled the identification of two heading-date QTL, named qHD1b and qHD7, of which the first was selected for further research. High-resolution linkage analysis was performed in BC5F4:5 and BC5F6 populations, and the location of qHD1b was confined to a 112.7-kb interval containing 17 predicted genes. Five of these genes contained polymorphisms in the promoter or coding regions and were thus considered as candidates. Expression analysis revealed a positive association between LOC_Os01g11940 expression and early heading. This locus was annotated as OsFTL1, which encodes an ortholog of Arabidopsis Flowering Locus T and was the most likely candidate gene for qHD1b. Our study revealed that qHD1b acts as a floral activator that promotes flowering by up-regulating Ehd1, Hd3a, RFT1, OsMADS14, and OsMADS15 under both short -day and long-day conditions. Field experiments showed that qHD1b affected several yield-related agronomic traits including 1000-grain weight and grain length. qHD1b could be useful for marker-assisted selection and breeding of early-maturing cultivars. (C) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.