The stimulation of bovine caudal epididymal sperm forward motility by bovine cumulus-egg complexes in vitro.
The stimulation of bovine caudal epididymal sperm forward motility by bovine cumulus-egg complexes in vitro.
复制标题
体外牛卵丘复合体刺激牛尾部附睾精子向前运动。
DOI：
10.1016/s0006-291x(83)80002-3
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Garbers,DL
中科院分区：
文献类型：
--
作者：
Bradley,MP;Garbers,DL
本文研究了卵丘-卵复合体、分离卵和卵丘细胞对牛精子前向运动的影响。在卵丘-卵复合体中孵育精子，可提高精子的前向运动指数。随后，研究表明，这些复合物可以释放刺激精子运动的物质到培养基中。FSH似乎引起卵丘-卵复合体释放更多的刺激物质。卵丘细胞与卵的分离表明刺激因子是从卵丘细胞而不是卵中释放的。这些结果表明，牛卵丘细胞与卵细胞可以影响精子的运动。
We have determined the effects of cumulus oophorus-egg complexes, and of separated eggs and cumulus oophorus cells on bovine sperm forward motility. The forward motility index ofspermatozoawas elevated by the incubation of spermatozoa in the presence of cumulus oophorus-egg complexes. Subsequently, it was shown that these complexes could release substance(s) into the medium which stimulated sperm motility. FSH appeared to cause a greater release of the stimulatory substance(s) from the cumulus oophorus-egg complexes. Separation of cumulus oophorus cells from eggs demonstrated that the stimulatory factor(s) were being released from the cumulus oophorus cells and not the eggs. These results demonstrate that bovine cumulus oophorus cells associated with the egg can influence spermatozoan motility.