AIM1 ‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice
AIM1 ‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice
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AIM1 依赖的高基础水杨酸积累调节水稻气孔孔径
DOI：
10.1111/nph.18842
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发表时间：
2023
期刊：
影响因子：
9.4
通讯作者：
Chen, Zhixiang
中科院分区：
文献类型：
--
作者：
Xu, Lei;Zhao, Hongyu;Wang, Junbin;Wang, Xuming;Jia, Xianqing;Wang, Long;Xu, Zhuang;Li, Ruili;Jiang, Kun;Chen, Zhixiang
水杨酸 (SA) 的基础水平在植物种类之间差异很大。例如，水稻芽中的 SA 含量几乎是拟南芥的 100 倍。尽管其基础水平较高，但水稻中SA的生物合成途径和生物学功能尚不清楚。结合代谢物分析、生理学和遗传学方法，我们发现水稻地上部基础SA的合成依赖于OsAIM1，OsAIM1编码苯丙氨酸解氨酶(PAL)途径中的β-氧化酶。Osaim1突变体中SA积累受损导致地上部温度低于野生型植物。然而，这种芽温度缺陷是由于突变体稳态气孔孔径增加导致蒸腾增加所致。此外，OsWRKY45的持续表达需要高基础SA水平，以调节水稻稳态气孔孔径和地上部温度。总之，这些结果为PAL途径在水稻高基础水平SA生物合成中的关键作用提供了直接的遗传证据，其在调节稳态气孔孔径以促进逆境条件下的适应性中发挥着重要作用。
The basal levels of salicylic acid (SA) vary dramatically among plant species. In the shoot, for example, rice contains almost 100 times higher SA levels than Arabidopsis. Despite its high basal levels, neither the biosynthetic pathway nor the biological functions of SA are well understood in rice.Combining with metabolite analysis, physiological, and genetic approaches, we found that the synthesis of basal SA in rice shoot is dependent onOsAIM1, which encodes a beta‐oxidation enzyme in the phenylalanine ammonia‐lyase (PAL) pathway.Compromised SA accumulation in theOsaim1mutant led to a lower shoot temperature than wild‐type plants. However, this shoot temperature defect resulted from increased transpiration due to elevated steady‐state stomatal aperture in the mutant. Furthermore, the high basal SA level is required for sustained expression ofOsWRKY45to modulate the steady‐state stomatal aperture and shoot temperature in rice.Taken together, these results provide the direct genetic evidence for the critical role of the PAL pathway in the biosynthesis of high basal level SA in rice, which plays an important role in the regulation of steady‐state stomatal aperture to promote fitness under stress conditions.