Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis)
Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis)
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绿茶（Camellia Sinensis）不同组分的多糖缀合物的抗氧化活性
DOI：
10.1016/j.foodchem.2007.06.040
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发表时间：
2008-01-15
期刊：
影响因子：
8.8
通讯作者：
Xie, Bijun
中科院分区：
文献类型：
--
作者：
Chen, Haixia;Zhang, Min;Xie, Bijun
采用吸附色谱法和离子交换色谱法从低档绿茶（Camellia sinensis）中分离纯化了水溶性多糖缀合物TPC-1、TPC-2和TPC-3。采用化学法、气相色谱法和激光光散射粒径排除色谱法测定了它们的化学和物理特性。采用脱氧核糖法、硝基蓝四唑光还原法和脂质过氧化抑制法检测茶多糖缀合物的体外抗氧化活性。结果表明，三种多糖缀合物均为与蛋白质结合的杂多糖。TPC-1由l -阿拉伯糖（Ara）、d -核糖（Rib）、d -木糖（Xyl）、d -葡萄糖（Gle）、d -半乳糖（Gal）和d -甘露糖（Man）组成。TPC-2仅由Ara、Rib、Glc和Man四种单糖组成。TPC-3中未检测到Man。TPC-1、TPC-2和TPC-3蛋白含量分别为2.8%、3.8%和4.0%，分子量分别为26.8 × 10(4)、11.8 × 10(4)和4.2 × 10(4)。TPC-3的抗氧化活性最高，脱氧核苷的IC50为182 μ g/ml，光还原NBT的IC50为93 μ g/ml，均低于TPC-1和TPC-2 （P < 0.01）。多糖偶联物的分子量和蛋白质含量对其生物活性的改善作用是显著的。(C) 2007 Elsevier Ltd.版权所有。
Three fractions of water-soluble polysaccharide conjugates, coded as TPC-1, TPC-2, and TPC-3, were isolated and purified from lowgrade green tea (Camellia sinensis) by absorbent chromatography and ion exchange chromatography. Their chemical and physical characteristics were determined by chemical methods, gas chromatography and size exclusion chromatography with laser light scattering. Deoxyribose assay, photoreduction of Nitro Blue Tetrazolium (NBT) assay and lipid peroxidation inhibition assay were applied to test the antioxidant activities of tea polysaccharide conjugates in vitro. The results indicated that the three polysaccharide conjugates were heteropolysaccharides bounded with protein. TPC-1 was composed Of L-arabinose (Ara), D-ribose (Rib), D-Xylose (Xyl), D-glucose (Gle), D-galactose (Gal) and D-mannose (Man). TPC-2 was only composed of four monosaccharides: Ara, Rib, Glc and Man. There was no Man detected in TPC-3. The protein contents of TPC-1, TPC-2, and TPC-3 were 2.8%, 3.8%, and 4.0% and the molecular weights were 26.8 x 10(4), 11.8 X 10(4), 4.2 x 10(4), respectively. TPC-3 showed the highest antioxidant activities among the three fractions of polysaccharide conjugates, with an IC50 of 182 mu g/ml for the deoxyribose assay, and 93 mu g/ml for the photoreduction of NBT assay, values which were lower than those of TPC-1 and TPC-2 (P < 0.01). The effects of the molecular weight and protein content of the polysaccharide conjugates on the improvement of the bioactivities appeared to be significant. (C) 2007 Elsevier Ltd. All rights reserved.