Tumour necrosis factor-alpha levels in aqueous humour and serum from patients with uveitis:: the involvement of HLA-B27
Tumour necrosis factor-alpha levels in aqueous humour and serum from patients with uveitis:: the involvement of HLA-B27
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DOI：
10.1185/030079903125002847
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发表时间：
2004-01-01
影响因子：
2.3
通讯作者：
Collantes-Estévez, E
中科院分区：
文献类型：
--
作者：
Pérez-Guijo, V;Santos-Lacomba, M;Collantes-Estévez, E
目的：为了研究局部和全身水平的肿瘤坏死因子-α的患者与活动性葡萄膜炎，并确定TNF-α在风湿性葡萄膜炎的影响，并观察这种关系是否是更显着的B27阳性patients.Patients和方法：选择患者的基础上的任何病因的葡萄膜炎的诊断。根据有无全身性风湿性疾病，将23例患者的数据分为两类。第一组包括9例风湿性疾病患者;第二组包括14例无风湿性疾病患者。这些患者还被细分为HLA-B27阳性（14例）和非HLA-B27阳性。将16名无并发症白内障患者的血清和房水中的TNF-α水平作为对照组进行分析。结果：对照组（n = 16）的血清TNF-α浓度为13.1 +/- 2.9 pg/ml，房水中的TNF-α浓度为0.56 +/- 1.53 pg/ml。在葡萄膜炎患者（n = 23）中，血清TNF-α浓度为35.35 +/-26.77 pg/ml，并且眼房水TNF-α浓度为15.1 +/-1.70 pg/ml（p < 0.01）。在HLA-B27阳性患者（n = 9）中，血清TNF-α浓度为45.56 +/- 34.17 pg/ml，并且眼房水TNF-α浓度为15.89 +/- 0.93 pg/ml。在HLA-B27阴性患者（n = 14）中，血清TNF-α浓度为28.79 +/- 19.38 pg/ml，房水TNF-α浓度为14.57 +/- 1.91 pg/ml（p < 0.01）。HLA-B27阳性患者的房水中TNF-α的浓度显著高于B27阴性患者。在有或无风湿性疾病的患者中，血清或房水中TNF-α的浓度无显著差异。TNF-α是一种可能积极参与临床葡萄膜炎发病机制的细胞因子。
Objective: To study the local and systemic levels of the tumour necrosis factor-alpha in patients with active uveitis and to determine the implication of TNF-alpha in rheumatological uveitis and to observe if this relationship is more significant in the B27 positive patients.Patients and methods: Patients were selected on the basis of a diagnosis of uveitis of any aetiology. Data from 23 patients were stratified into two categories according to the presence or absence of systemic rheumatic disease. The first group comprised nine patients with rheumatic disease; the second group contained 14 patients without rheumatic disease. The patients were also sub-classified into those who were HLA-B27 positive (14 patients) and those who were not. TNF-alpha levels in serum and aqueous humour from a group of 16 patients with uncomplicated cataracts were analysed as a control group.Results: In the control group (n = 16) the serum TNF-alpha concentration was 13.1 +/- 2.9 pg/ml and the aqueous humour concentration of TNF-alpha was 0.56 +/- 1.53 pg/ml. In uveitis patients (n = 23) the serum TNF-alpha concentration was 35.35 +/- 26.77 pg/ml and the aqueous humour concentration of TNF-alpha was 15.1 +/- 1.70 pg/ml (p < 0.01). In HLA-B27 positive patients (n = 9) the serum TNF-alpha concentration was 45.56 +/- 34.17 pg/ml and the aqueous humour concentration of TNF-alpha was 15.89 +/- 0.93 pg/ml. In HLA-B27 negative patients (n = 14) the serum TNF-alpha concentration was 28.79 +/- 19.38 pg/ml and aqueous humour concentration of TNF-alpha was 14.57 +/- 1.91 pg/ml (p < 0.01).Conclusions: The concentration of TNF-alpha in aqueous humour in patients who are HLA-B27 positive is significantly greater than in those who are B27 negative. No significant differences in the concentrations of TNF-alpha in serum or aqueous humour in patients with or without rheumatic diseases were detected. TNF-alpha is a cytokine that may participate actively in the pathogenesis of clinical uveitis.