Interactions among iron(II) bleomycin, Lewis bases, and DNA.
Interactions among iron(II) bleomycin, Lewis bases, and DNA.
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博莱霉素铁 (II)、路易斯碱基和 DNA 之间的相互作用。
DOI：
10.1073/pnas.78.12.7517
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发表时间：
1981
影响因子：
11.1
通讯作者：
Brown,CE
中科院分区：
文献类型：
--
作者：
Antholine,WE;Petering,DH;Saryan,LA;Brown,CE
通过比较含硫配体与Fe（II）博莱霉素[Fe（II）Blm]配合物的DNA断裂活性和配合物的结构特征，研究了含硫配体与配合物的相互作用。过量的Blm的双噻唑环可以与Fe（II）Blm和NO-Fe（II）Blm形成分子间配合物，并且CO-Fe（II）Blm的双噻唑基团与金属中心相互作用。这种结合可以被谷胱甘肽逆转，CO-Fe（II）Blm结合到聚（dA-dT）。聚（dA-dT）通过其联噻唑基团改变Fe 2+离子的环境。谷氨酸与CO-Fe（II）Blm-poly（dA-dT）结合。聚（dA-dT）复合物的条件下，它刺激DNA链断裂的Fe（II）Blm。
The interaction of sulfur-containing ligands with complexes of Fe(II) bleomycin [Fe(II)Blm] was investigated by comparing DNA strand-scission activity with the structural characteristics of the complex. The bithiazole ring of excess Blm can form intermolecular complexes with Fe(II)Blm and with NO-Fe(II)Blm, and the bithiazole groups of CO-Fe(II)Blm interact with the metal center. This binding can be reversed by glutathione, CO-Fe(II)Blm binds to poly(dA-dT) . poly(dA-dT) via its bithiazole group to change the environment of the Fe2+ ion. Glutathione binds to the CO-Fe(II)Blm-poly(dA-dT) . poly(dA-dT) complex under conditions in which it stimulates DNA strand scission by Fe(II)Blm.