Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes
Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes
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DOI：
10.1101/gad.367606
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发表时间：
2006-02-15
影响因子：
10.5
通讯作者：
Breitbart, H
中科院分区：
文献类型：
--
作者：
Gur, Y;Breitbart, H
人们普遍认为精子在翻译上是沉默的。本研究首次证明了在精子获能过程中，标记氨基酸被掺入多肽中，这一过程可被线粒体翻译抑制物完全抑制，但不能被细胞质翻译抑制物抑制。与80S细胞质核糖体不同，55S线粒体核糖体存在于多体组分中，表明这些核糖体积极参与精子中的蛋白质翻译。抑制蛋白质翻译显著降低精子活动率、获能能力和体外受精率。因此，与公认的教条相反，核基因在精子中以蛋白质的形式表达，在受精之前驻留在女性生殖道中。
It is widely accepted that spermatozoa are translationally silent. The present study demonstrates, for the first time, incorporation of labeled amino acids into polypeptides during sperm capacitation, which was completely inhibited by mitochondrial translation inhibitors but not by the cytoplasmic translation inhibitor. Unlike 80S cytoplasmic ribosomes, 55S mitochondrial ribosomes were present in polysomal fractions, indicating that these ribosomes are actively involved in protein translation in spermatozoa. Inhibition of protein translation significantly reduced sperm motility, capacitation and in vitro fertilization rate. Thus, contrary to the accepted dogma, nuclear genes are expressed as proteins in sperm during their residence in the female reproductive tract until fertilization.