Survival Rate, Chemical and Microbial Properties of Oak Seedlings Planted with or without Oak Forest Soils in a Black Locust Forest of a Dryland
Survival Rate, Chemical and Microbial Properties of Oak Seedlings Planted with or without Oak Forest Soils in a Black Locust Forest of a Dryland
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DOI：
10.3390/f12060669
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发表时间：
2021-05
期刊：
影响因子：
2.9
通讯作者：
Chikae Tatsumi;T. Taniguchi;F. Hyodo;Sheng Du;N. Yamanaka;R. Tateno
中科院分区：
文献类型：
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作者：
Chikae Tatsumi;T. Taniguchi;F. Hyodo;Sheng Du;N. Yamanaka;R. Tateno
本土树种往往无法有效地生长在非本土树木的种植方案。在黄土高原地区，在非原生刺槐林中很难找到原生的辽东栎幼苗。刺槐是一种丛枝菌根（AM）树，而橡树是一种外生菌根（ECM）树。植物高度依赖于它们的共生菌根真菌来吸收水、氮（N）和其他营养物质。我们假设，刺槐林不会提供ECM接种橡树幼苗，限制他们的水分和养分的吸收，这将改善ECM接种。在这里，我们（1）播种种子，有或没有橡树林土壤，（2）移植幼苗收集在橡树林，有或没有橡树林土壤，（3）种植幼苗发芽高压灭菌或未高压灭菌的橡树林土壤。我们测量了所有三个实验的存活率和生长率，沿着化学性质和根ECM定殖。橡树林土壤播种的栎类种子的菌根定殖率、叶片N浓度和存活率均高于不播种橡树林土壤的栎类种子，而根系δ 13 C则低于不播种的栎类种子。种植橡树林土壤也增加了发芽苗的成活率，但不是移植苗。总体而言，我们的研究表明，在刺槐林中使用橡树林土壤，通过提供ECM接种物来提高橡树幼苗对水和氮的吸收，从而获得较高的存活率。我们的研究还表明，与移植野生/苗圃幼苗相比，播种种子的方法是轻松有效的。
Native tree species are frequently unable to effectively grow in non-native tree cultivation scenarios. In the Loess Plateau, China, it is difficult to find native oak (Quercus liaotungensis) seedlings in non-native black locust forests. Black locust is an arbuscular mycorrhizal (AM) tree, but oak is an ectomycorrhizal (ECM) tree. Plants highly depend on their symbiotic mycorrhizal fungi to take up water, nitrogen (N) and other nutrients. We hypothesized that black locust forests would not provide ECM inoculum to oak seedlings, limiting their water and nutrient uptake, which would be improved by ECM inoculum. Here, we (1) sowed seeds, with or without oak forest soils, (2) transplanted seedlings collected in oak forests, with or without oak forest soils, and (3) planted seedlings germinated in autoclaved or unautoclaved oak forest soils. We measured the survival and growth rate for all three experiments, along with chemical properties, and root ECM colonization. Oak seeds sowed with oak forest soils had higher mycorrhizal colonization, leaf N concentrations and survival rate, and lower root δ13C than the seeds sowed without oak forest soils. Planting with oak forest soils also increased the survival rate of the germinated seedlings, but not the transplanted seedlings. Overall, our study suggested that the use of oak forest soils in the black locust forest to improve the water and N uptake of oak seedlings by providing the ECM inoculum, resulting in a high survival rate. Our study also implies that the method of sowing seeds was effortless and effectively compared to transplanting wild/nursery seedlings.