Simultaneous T2* mapping of 14N- and 15N-labeled dicarboxy-PROXYLs using CW-EPR-based single-point imaging
Simultaneous T2* mapping of 14N- and 15N-labeled dicarboxy-PROXYLs using CW-EPR-based single-point imaging
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DOI：
10.1016/j.jmr.2019.06.012
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发表时间：
2019-08-01
影响因子：
2.2
通讯作者：
Hirata, Hiroshi
中科院分区：
文献类型：
--
作者：
Yokoyama, Takahito;Taguchi, Akihiro;Hirata, Hiroshi
本文报道了一种利用750 mhz连续波电子顺磁共振（CW-EPR）成像同时对N-14和n -15标记的二羧基proxyl进行T-2*定位的方法。为了分离磁场梯度下N-14和n -15标记的二羧基proxyls的光谱，提出了以空间总变分为正则化项的光谱投影优化问题，并采用基于梯度下降算法的局部搜索进行求解。采用单点成像（SPI）方法，对每个自由基进行光谱投影，同时对溶液样品进行T-2*映射。同时T-2*映射使T-2*值对溶液中溶解氧水平的响应可视化。同时应用于小鼠肿瘤模型的T-2*映射证明了该方法在体内氧合成像中的潜在应用可行性。(C) 2019 Elsevier Inc.版权所有。
This article reports a method of simultaneous T-2* mapping of N-14- and N-15-labeled dicarboxy-PROXYLs using 750-MHz continuous-wave electron paramagnetic resonance (CW-EPR) imaging. To separate the spectra of N-14- and N-15-labeled dicarboxy-PROXYLs under magnetic field gradients, an optimization problem for spectral projections was formulated with the spatial total variation as a regularization term and solved using a local search based on the gradient descent algorithm. Using the single-point imaging (SPI) method with spectral projections of each radical, simultaneous T-2* mapping was performed for solution samples. Simultaneous T-2* mapping enabled visualization of the response of T-2* values to the level of dissolved oxygen in the solution. Simultaneous T-2* mapping applied to a mouse tumor model demonstrated the feasibility of the reported method for potential application to in vivo oxygenation imaging. (C) 2019 Elsevier Inc. All rights reserved.