Sex Steroid and Thyroid Hormone Receptor Expressions in the Thyroid of the American Alligator (ALLIGATOR MISSISSIPPIENSIS) During Different Life Stages
Sex Steroid and Thyroid Hormone Receptor Expressions in the Thyroid of the American Alligator (ALLIGATOR MISSISSIPPIENSIS) During Different Life Stages
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DOI：
10.1002/jmor.10936
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发表时间：
2011-06-01
影响因子：
1.5
通讯作者：
Guillette, Louis J., Jr.
中科院分区：
文献类型：
--
作者：
Bermudez, Dieldrich S.;Skotko, Jeremy P.;Guillette, Louis J., Jr.
在除少数哺乳动物外的少数脊椎动物物种中，已报道了甲状腺中雌激素受体ESR 1（ER α）和ESR 2（ER β）以及雄激素受体（AR）的表达。这项研究报告了性类固醇激素受体和甲状腺受体（ER α，ER β，AR，TR α和TR β）在美国短吻鳄的甲状腺在几个生命阶段的存在。它使用免疫组织化学方法提供了甲状腺滤泡细胞中ER α的半定量和分布，并使用定量真实的时间-PCR（Q-PCR）和专为短吻鳄设计的引物报告了同一组织中ER α、ER β、TR α、TR β和AR mRNA表达的定量差异。美洲鳄的甲状腺组织表达ER α，ER β和AR在所有的生命阶段，虽然没有统计学上的显着差异，观察到男性和女性之间的甲状腺mRNA表达的任何基因分析。雌激素受体α免疫组化染色未见性别二型性。由于季节的混杂因素，未进行各生命阶段的统计分析。J. Morphol. 272：698-703，2011。(C)2011 Wiley-Liss，Inc.
The expression of estrogen receptors, ESR1 (ER alpha) and ESR2 (ER beta), and androgen receptors (AR) in the thyroid gland has been reported in few vertebrate species other than a few mammals. This study reports the presence of sex steroid hormone receptors and thyroid receptors (ER alpha, ER beta, AR, TR alpha, and TR beta) in the thyroid gland of the American alligator at several life stages. It provides a semiquantification and distribution of ER alpha in the thyroid follicle cells using an immunohistochemical approach as well as reports quantitative differences in mRNA expression of ER alpha, ER beta, TR alpha, TR beta, and AR in the same tissue using quantitative real time-PCR (Q-PCR) with primers designed specifically for alligators. The thyroid tissue of the American alligator expresses ER alpha, ER beta, and AR at all of the life stages examined here although no statistically significant differences were observed between male and female in thyroid mRNA expression for any of the genes analyzed. No sexual dimorphism was observed in ER alpha immunostaining. No statistical analysis across life stages were performed due to confounding factor of season. J. Morphol. 272: 698-703, 2011. (C) 2011 Wiley-Liss, Inc.