Study of active substances involved in skin dysfunction induced by crowding stress. I. Effect of crowding and isolation on some physiological variables, skin function and skin blood perfusion in hairless mice
Study of active substances involved in skin dysfunction induced by crowding stress. I. Effect of crowding and isolation on some physiological variables, skin function and skin blood perfusion in hairless mice
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DOI：
10.1248/bpb.26.170
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发表时间：
2003-02-01
影响因子：
2
通讯作者：
Tsuji, K
中科院分区：
文献类型：
--
作者：
Ishida, H;Mitsui, K;Tsuji, K
研究了5种不同密度水平的无毛小鼠各器官、神经内分泌系统、皮肤功能、皮肤血液灌注量和血液参数的影响。通过测量胶带剥离后的经皮水分损失来评估皮肤屏障的恢复情况。用激光多普勒成像技术测量血液灌注量。在拥挤的动物模型中，还检测了副交感神经刺激剂氯化卡普林对皮肤功能的影响。与对照组(5只/笼)相比，拥挤7d(10、15、20只/笼)小鼠的血皮质酮、儿茶酚胺(去甲肾上腺素、肾上腺素和多巴胺)、血糖和血清乳酸脱氢酶活性显著升高，肾、卵巢和胸腺萎缩，肾上腺肥大，体重增加减少。拥挤还增加了表皮厚度和表皮增殖活性，减少了角质细胞大小、屏障恢复率和皮肤血液灌注量。随着人口密度的增加和/或更长时间地暴露在拥挤的环境中，这些变化大多变得更加明显。隔离(1只小鼠/笼)可增加去甲肾上腺素水平和皮肤血液灌注率，并显著延迟屏障恢复。重复局部应用卡普因7d可改善拥挤所致的皮肤血液灌注量、屏障恢复、肾脏和卵巢以及表皮形态的改变。拥挤动物模型可用于客观量化拥挤环境应激对皮肤功能和血液灌注量的影响。
The effects of five levels of population density on various organs, the neuroendocrine system, skin function, skin blood perfusion, and blood parameters were studied in the hairless mouse. Skin barrier recovery was evaluated by measuring transepidermal water loss after tape stripping. Blood perfusion was measured by means of a laser Doppler imaging technique. The effect of a parasympathetic nerve stimulator, carpronium chloride, on skin function in the crowded animal model was also examined. A 7 d crowding (10, 15, 20 mice/cage) significantly increased the levels of corticosterone, catecholamines (norepinephrine, epinephrine and dopamine), glucose and serum lactate dehydrogenase activity in circulating blood, induced atrophy of kidney, ovary and thymus and hypertrophy of adrenal glands, and decreased body weight gain in comparison with the control (5 mice/cage). Crowding also increased epidermal thickness and epidermal proliferative activity, and decreased corneocyte size, rate of barrier recovery and skin blood perfusion. Most of these changes became more marked with increasing population density and/or longer exposure to a crowded environment. Isolation (1 mouse/cage) increased the level of norepinephrine and rate of skin blood perfusion, and significantly delayed barrier recovery. Repeated topical applications of carpronium chloride for 7 d improved the changes in skin blood perfusion, barrier recovery, kidney and ovary, and epidermal morphology induced by crowding. The crowded animal model could be useful for quantifying objectively the influence of crowded environment-induced stress on cutaneous function and blood perfusion.