A Nanoplasmonic Portable Molecular Interaction Platform for High-Throughput Drug Screening
A Nanoplasmonic Portable Molecular Interaction Platform for High-Throughput Drug Screening
复制标题
用于高通量药物筛选的纳米等离子体便携式分子相互作用平台
DOI：
10.1002/adfm.202203635
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发表时间：
2022-09-01
影响因子：
19
通讯作者：
Liu, Gang L.
中科院分区：
文献类型：
--
作者：
Fan, Hongli;Huang, Liping;Liu, Gang L.
用于实时检测分子相互作用的快速可靠方法对于药物筛查和控制感染或疾病是必需的。因此，本研究旨在开发高通量便携式纳米质平台。这是通过与标准的96孔板或简单的八柱设备集成纳米级阵列增强表面等离子体共振（NANOSPR）传感器来完成的，该传感器可以提供快速且无标记的相互作用分析，并提供高度敏感的结合动力学来进行药物筛查。为了制造晶圆级纳米型生物传感器，纳米印刷光刻，电子束蒸发和粘合技术已被用于以质量规模提供低成本生产。同时，各种现成的纳米PR生物传感器进行了分别修改，以证明不同生物分子相互作用的高质量结合动力学和亲和力。由于其独特的光学特性，这两个开发的便携式NanoSPR设备提供了一种便携式台式分子交互仪器的可能性，这对于满足个人和实验室测试的需求非常有希望。这项工作可能为纳米PR生物传感器铺平了道路，以实现具有出色性能的高通量和易于使用的分子交互分析。
A fast and reliable method for the detection of molecular interactions in real-time is necessary for drug screening and control of infections or diseases. Therefore, this study aims to develop a high-throughput portable nanoplasmonic platform. This is done by integrating a nanocup array-enhanced surface plasmon resonance (NanoSPR) sensor with a standard 96-well plate or a simple eight-pillar device, which can offer rapid and label-free interaction analysis and highly sensitive binding kinetics for drug screening. To fabricate the wafer-level NanoSPR biosensor, nanoimprint lithography, electron beam evaporation, and bonding technology have been used to offer low-cost production on a mass scale. Meanwhile, a variety of ready-to-use NanoSPR biosensors are separately modified to demonstrate the high-quality binding kinetics and affinity of different biomolecular interactions. Owing to their unique optical properties, the two developed portable NanoSPR devices provide the possibility of having a portable benchtop molecular interaction instrument that is very promising for meeting the needs of personal and laboratory testing. This work may pave the way for NanoSPR biosensors to achieve high-throughput and easy-to-use molecular interaction analysis with outstanding performance.