LKB1 destabilizes microtubules in myoblasts and contributes to myoblast differentiation.
LKB1 destabilizes microtubules in myoblasts and contributes to myoblast differentiation.
复制标题
DOI：
10.1371/journal.pone.0031583
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tirnauer JS
中科院分区：
文献类型：
--
作者：
Mian I;Pierre-Louis WS;Dole N;Gilberti RM;Dodge-Kafka K;Tirnauer JS
Skeletal muscle myoblast differentiation and fusion into multinucleate myotubes is associated with dramatic cytoskeletal changes. We find that microtubules in differentiated myotubes are highly stabilized, but premature microtubule stabilization blocks differentiation. Factors responsible for microtubule destabilization in myoblasts have not been identified. We find that a transient decrease in microtubule stabilization early during myoblast differentiation precedes the ultimate microtubule stabilization seen in differentiated myotubes. We report a role for the serine-threonine kinase LKB1 in both microtubule destabilization and myoblast differentiation. LKB1 overexpression reduced microtubule elongation in a Nocodazole washout assay, and LKB1 RNAi increased it, showing LKB1 destabilizes microtubule assembly in myoblasts. LKB1 levels and activity increased during myoblast differentiation, along with activation of the known LKB1 substrates AMP-activated protein kinase (AMPK) and microtubule affinity regulating kinases (MARKs). LKB1 overexpression accelerated differentiation, whereas RNAi impaired it. Reduced microtubule stability precedes myoblast differentiation and the associated ultimate microtubule stabilization seen in myotubes. LKB1 plays a positive role in microtubule destabilization in myoblasts and in myoblast differentiation. This work suggests a model by which LKB1-induced microtubule destabilization facilitates the cytoskeletal changes required for differentiation. Transient destabilization of microtubules might be a useful strategy for enhancing and/or synchronizing myoblast differentiation.
登录
查看更多内容
影响因子：
5.3
作者：
Asada, Naoyuki;Sanada, Kamon;Fukada, Yoshitaka
通讯作者：
Fukada, Yoshitaka
DOI：
10.1016/j.bpg.2009.02.007
发表时间：
2009-04-01
影响因子：
3.2
作者：
Gammon, Amanda;Jasperson, Kory;Burt, Randall W.
通讯作者：
Burt, Randall W.
影响因子：
4.8
作者：
Kojima, Yasushi;Miyoshi, Hiroyuki;Taketo, Makoto M.
通讯作者：
Taketo, Makoto M.
影响因子：
7.8
作者：
ANTIN, PB;FORRYSCHAUDIES, S;HOLTZER, H
通讯作者：
HOLTZER, H
影响因子：
3.3
作者：
Dorfman, Julia;Macara, Ian G.
通讯作者：
Macara, Ian G.