RSC unravels the nucleosome
RSC unravels the nucleosome
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DOI：
10.1016/s1097-2765(01)00157-5
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发表时间：
2001-01-01
期刊：
影响因子：
16
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Lorch, Y;Zhang, MC;Kornberg, RD
RSC和SWI/SNF染色质重塑复合物先前被报道产生稳定改变的核小体。我们现在描述了不同大小核小体之间杂交的形成，表明稳定改变的结构是非共价二聚体。二聚体形成的基础是由RSC对核小体封闭的圆形阵列的超卷曲的影响提出的。这种效应可以解释为RSC与核小体末端DNA的相互作用，这可能导致从末端释放60-80 bp或更多。以这种方式释放的DNA可能被困在稳定的二聚体中，或导致其他命运，如组蛋白八聚体转移到另一个DNA或沿着相同的DNA分子滑动。
RSC and SWI/SNF chromatin-remodeling complexes were previously reported to generate a stably altered nucleosome. We now describe the formation of hybrids between nucleosomes of different sizes, showing that the stably altered structure is a noncovalent dimer. A basis for dimer formation is suggested by an effect of RSC on the supercoiling of closed, circular arrays of nucleosomes. The effect may be explained by the interaction of RSC with DNA at the ends of the nucleosome, which could lead to the release 60-80 bp or more from the ends. DNA released in this way may be trapped in the stable dimer or lead to alternative fates such as histone octamer transfer to another DNA or sliding along the same DNA molecule.