Injectable Gel Constructs with Regenerative and Anti-Infective Dual Effects Based on Assembled Chitosan Microspheres
Injectable Gel Constructs with Regenerative and Anti-Infective Dual Effects Based on Assembled Chitosan Microspheres
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基于组装壳聚糖微球的具有再生和抗感染双重功效的注射凝胶结构
DOI：
10.1021/acsami.8b06648
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发表时间：
2018-08-01
影响因子：
9.5
通讯作者：
Li, Yubao
中科院分区：
文献类型：
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作者：
Cai, Bin;Zou, Qin;Li, Yubao
在临床应用中，对既有助于骨再生又具有抗感染品质的生物材料的需求日益增加。为了实现这一目标，制备了带有正电荷或负电荷的壳聚糖微球，然后组装成用于骨愈合的凝胶。带正电荷的壳聚糖微球（CSM;类似于35.5 μ m）和带负电荷的O-羧甲基壳聚糖微球（CMCSM;类似于13.5 μ m），分别与骨形态发生蛋白（BMP-2）和小檗碱（Bbr）通过溶胀包封和物理吸附，而没有显着的电荷变化。体外释放动力学的BMP-2和Bbr从微球进行了研究。结果表明，Bbr/CMCSM微球组对金黄色葡萄球菌具有较强的抗菌活性; BMP-2/CSM微球组在BMP-2的辅助下，具有良好的细胞相容性，并提高了骨诱导活性。由Bbr/CMCSM和BMP-2/CSM组成的组装凝胶组具有允许生物信号传递和组织浸润的多孔结构，并且与相应的微球组相比，显示出显著增强的骨重建，这应该是由于多孔结构的骨传导性和BMP-2生长因子的骨诱导。带相反电荷的微球及其组装的凝胶为制造具有再生和抗感染双重作用的可注射组织工程构建体用于生物医学应用提供了广阔的前景。
There is increasing demand for biomaterials that both assist with bone regeneration and have anti-infection qualities in clinical applications. To achieve this goal, chitosan microspheres with either positive or negative charges were fabricated and then assembled as a gel for bone healing. The positively charged chitosan microspheres (CSM; similar to 35.5 mu m) and negatively charged O-carboxymethyl chitosan microspheres (CMCSM; similar to 13.5 mu m) were loaded, respectively, with bone morphogenetic protein (BMP-2) and berberine (Bbr) via swollen encapsulation and physical adsorption without a significant change in the electric charges. The release kinetics of BMP-2 and Bbr from the microspheres were also studied in vitro. The results showed that the Bbr/CMCSM microsphere group possessed high antibacterial activity against Staphylococcus aureus; the BMP-2/CSM microsphere group also had excellent cytocompatibility and improved osteoinductivity with the assistance of BMP-2. The assembled gel group consisting of Bbr/CMCSM and BMP-2/CSM had a porous structure that allowed biological signal transfer and tissue infiltration and exhibited significantly enhanced bone reconstruction compared with that of the respective microsphere groups, which should result from the osteoconductivity of the porous structure and the osteoinduction of the BMP-2 growth factor. The oppositely charged microspheres and their assembled gel provide a promising prospect for making injectable tissue-engineered constructs with regenerative and anti-infective dual effects for biomedical applications.