Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation
Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation
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DOI：
10.1016/s0968-0004(99)01535-2
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发表时间：
2000-03-01
影响因子：
13.8
通讯作者：
Orlando, V
中科院分区：
文献类型：
--
作者：
Orlando, V
基因调控是一个复杂的过程。每个基因精确的时间和空间调控似乎需要许多因素。这些因子通常被组装成多蛋白复合物，参与特定的基因调控事件。了解所有这些因子如何在染色体中组织以及它们的功能如何在体内被调节是一项具有挑战性的任务。研究这一水平基因调控的最有用的技术之一是用甲醛将蛋白质与蛋白质和蛋白质与DNA交联进行体内固定，然后对固定材料进行免疫沉淀。
Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events, Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material.