Can intermediate-frequency magnetic fields affect memory function-related gene expressions in hippocampus of C57BL/6J mice?
Can intermediate-frequency magnetic fields affect memory function-related gene expressions in hippocampus of C57BL/6J mice?
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DOI：
10.2131/jts.38.169
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发表时间：
2013-04-01
影响因子：
2
通讯作者：
Kunugita, Naoki
中科院分区：
文献类型：
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作者：
Win-Shwe, Tin-Tin;Ohtani, Shin;Kunugita, Naoki
近来，基于电磁感应原理的烹饪器具已经在国内广泛使用;这种感应加热烹饪灶具主要产生中频磁场（IF-MF）。然而，家用电器产生的电磁场是否会对健康构成风险仍不确定。本研究探讨了干扰素-微乳对小鼠海马记忆功能相关基因及相关转导分子表达的影响。将雄性和雌性C57 BL/6 J小鼠分配到对照组（假暴露）、暴露组或恢复组（暴露后1周），并每天暴露于IF-MF（21 kHz，3.8 mT）1小时，持续2周。在最终暴露后24小时，使用实时RT-PCR检测海马中记忆功能相关基因的表达水平和信号转导通路分子的mRNA水平。在IF-MF暴露小鼠中，N-甲基-D天冬氨酸（NMDA）受体亚基NR 1、NR 2A和NR 2B以及转录因子（钙/钙调蛋白依赖性蛋白激酶（CaMK）-IV、环AMP反应元件结合蛋白（CREB）-1）和神经营养因子（神经生长因子（NGF）和脑源性神经营养因子（BDNF））的相对mRNA表达水平没有显著改变。我们还检查了。使用组织学分析观察海马的形态，但在IF-MF暴露的小鼠中没有观察到变化。这是第一项体内研究，表明IF-MF暴露不会影响C57 BL/6 J小鼠海马中记忆功能相关基因的表达水平。目前的研究结果表明，IF-MF暴露可能不会影响本动物模型的认知功能。
Recently, a cooking appliance based on the principle of electromagnetic induction has come to be used domestically on a widespread basis; this induction heating cooking hob mainly generates intermediate-frequency magnetic fields (IF-MF). However, whether electromagnetic fields originating from household appliances represent a health risk remains uncertain. We investigated the effect of IF-MF on the expressions of memory function-related genes and related transduction molecules in the mouse hippocampus. Male and female C57BL/6J mice were allotted to a control (sham-exposed), an exposure, or a recovery (one week after exposure) group and were exposed to IF-MF (21 kHz, 3.8 mT) one hour per day for 2 weeks. Twenty-four hour after final exposure, the expression levels of memory function-related genes and the mRNA levels for signal transduction pathway molecules in the hippocampi were examined using real-time RT-PCR. The relative mRNA expression levels of the N-methyl-D aspartate (NMDA) receptor subunits NR1, NR2A, and NR2B as well as transcription factors (calcium/calmodulin-dependent protein kinase (CaMK) -IV, cyclic AMP responsive element binding protein (CREB) -1) and neurotrophins (nerve growth factor (NGF), and brain-derived neurotrophic factors (BDNF)) were not significantly altered in the IF-MF-exposed mice. We also examined the. morphology of the hippocampus using a histological analysis, but no changes in the IF-MF-exposed mice were seen. This is the first in vivo study to show that IF-MF exposure did not affect the expression levels of memory function-related genes in the hippocampus of C57BL/6J mice. The present findings suggest that IF-MF exposure may not affect cognitive function in the present animal model.