Determination of triglyceride in the human myocardium by magnetic resonance spectroscopy: reproducibility and sensitivity of the method
Determination of triglyceride in the human myocardium by magnetic resonance spectroscopy: reproducibility and sensitivity of the method
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DOI：
10.1152/ajpendo.00095.2005
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发表时间：
2005-11-01
影响因子：
5.1
通讯作者：
Szczepaniak, LS
中科院分区：
文献类型：
--
作者：
Reingold, JS;McGavock, JM;Szczepaniak, LS
本研究的主要目的是确定H-1磁共振波谱（H-1-MRS）作为一种定量人体心肌甘油三酯（TG）含量的方法的可靠性和灵敏度。设计了三个独立的实验来量化心肌TG含量：1）90天内，2）高脂餐后，3）48小时禁食后。质子谱是从放置在心室间隔内的10 x 20 x 30 mm（3）体素中收集的，测量值是在收缩末期和呼气末期使用心脏触发和呼吸门控采集的。间隔90天测定的心肌TG含量之间观察到最小的变化（r = 0.98，CV = 5%），而TG值不受高脂餐的影响，尽管血清TG显著增加两倍（P < 0.05）。与此相反，尽管血清TG降低了25%，但禁食48小时后心肌TG含量增加了3倍（P < 0.05）。体重指数与心肌TG（r = 0.58，P < 0.05）及禁食48 h后心肌TG的变化（r（2）= 0.60）显著相关。H-1-MRS是测定人体心肌TG的可靠方法，相对不受一顿高脂餐的影响，但对长时间禁食后的变化敏感。
The primary aim of this investigation was to determine the reliability and sensitivity of H-1 magnetic resonance spectroscopy (H-1-MRS) as a method for quantifying myocardial triglyceride (TG) content in humans over time and in response to metabolic perturbations. Three separate experiments were designed to quantify myocardial TG content 1) over a 90-day period, 2) after a high-fat meal, and 3) after a 48-h fast. Proton spectra were collected from a 10 x 20 x 30-mm(3) voxel placed within the intraventricular septum, with measurements acquired at end-systole and end-expiration, using cardiac triggering and respiratory gating. Minimal variation was observed between myocardial TG content determined 90 days apart ( r = 0.98, CV = 5%), whereas TG values were unaffected by a high-fat meal despite a significant twofold increase ( P < 0.05) in serum TG. In contrast, myocardial TG content increased threefold ( P < 0.05) after a 48-h fast despite a 25% reduction in serum TG. Body mass index was significantly related to myocardial TG ( r = 0.58, P < 0.05) and the change in myocardial TG after a 48-h fast ( r(2) = 0.60). H-1-MRS is a reliable method for the determination of myocardial TG in humans and is relatively unaffected by the consumption of one high-fat meal but sensitive to changes following a prolonged fast.