Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis
Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis
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DOI：
10.1242/dev.01772
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发表时间：
2005-04-01
期刊：
影响因子：
4.6
通讯作者：
Drummond, IA
中科院分区：
文献类型：
--
作者：
Kramer-Zucker, AG;Olale, F;Drummond, IA
纤毛作为运动和感觉细胞器，与正常发育以及包括囊性肾病、脑积水和内脏逆位在内的疾病有关。在肾上皮细胞中，纤毛被认为是非运动的感觉细胞器，而在小鼠淋巴结中，两种纤毛群体，运动的和非运动的被认为是调节位置的。我们发现，纤毛在斑马鱼幼虫的肾脏，脊髓和Kupffer囊泡是能动的，这表明流体流动是一个共同的特点，这些器官。纤毛结构或运动的破坏导致前肾囊肿形成、脑积水和左右不对称缺陷。数据显示，流体流动的损失导致流体积聚，这可以解释肾脏和大脑中的器官扩张病理。在库普弗囊泡，流量的损失与左，右图案的损失，表明“节点流”机制产生的部位是保守的非哺乳类脊椎动物。
Cilia, as motile and sensory organelles, have been implicated in normal development, as well as diseases including cystic kidney disease, hydrocephalus and situs inversus. In kidney epithelia, cilia are proposed to be nonmotile sensory organelles, while in the mouse node, two cilia populations, motile and non-motile have been proposed to regulate situs. We show that cilia in the zebrafish larval kidney, the spinal cord and Kupffer's vesicle are motile, suggesting that fluid flow is a common feature of each of these organs. Disruption of cilia structure or motility resulted in pronephric cyst formation, hydrocephalus and left-right asymmetry defects. The data show that loss of fluid flow leads to fluid accumulation, which can account for organ distension pathologies in the kidney and brain. In Kupffer's vesicle, loss of flow is associated with loss of left-right patterning, indicating that the 'nodal flow' mechanism of generating situs is conserved in non-mammalian vertebrates.