Association between cag-pathogenicity island in Helicobacter pylori isolates from peptic ulcer, gastric carcinoma, and non-ulcer dyspepsia subjects with histological changes
Association between cag-pathogenicity island in Helicobacter pylori isolates from peptic ulcer, gastric carcinoma, and non-ulcer dyspepsia subjects with histological changes
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DOI：
10.3748/wjg.v11.i43.6815
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发表时间：
2005-11-21
影响因子：
4.3
通讯作者：
Habibullah, C. M.
中科院分区：
文献类型：
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作者：
Ali, Mahaboob;Khan, Aleem A.;Habibullah, C. M.
目的：探讨完全cog-PAI和部分缺失菌株的cag-致病性岛的存在及其与疾病状态的相关组织学损害。方法：采用8种不同的寡核苷酸引物cagA1、cagA2、cagAP1、cagAP2、cagE、cagT、lec1、lec2，采用聚合酶链反应（PCR）方法，对174株具有代表性的十二指肠溃疡、胃溃疡、胃癌和非溃疡性消化不良患者的幽门螺杆菌临床分离株的全cog-PAI进行分析，这些引物跨越全cog-PAL的5个不同位点。结果：cog-PAL组成基因的完全筛选结果显示，胃溃疡患者中存在完全cog-PAL的比例最大（97.8%），其次是胃癌（85.7%）、非溃疡性消化不良（7.1%）和十二指肠溃疡（6.9%），各临床组中基因的百分比分布差异有统计学意义。结果发现，具有完全cog-PAL的菌株比部分缺失的菌株能造成严重的组织损伤。结论：cog-PAI在疾病发病过程中是一个很强的毒力标志物，因为大量感染完全cag-PAI的菌株具有一种或另一种不可逆的胃病理，有趣的是，其中18.5%的人发展为胃癌。完整的cag-PAI的存在与更严重的病理发展相关，这种菌株在严重胃十二指肠疾病患者中更常见。cog-PAI的部分缺失似乎足以使生物体的致病性降低。(C) 2005 WJG出版社和爱思唯尔公司。版权所有。
AIM: To investigate the presence of the cag-pathogenicity island and the associated histological damage caused by strains with complete cog-PAI and with partial deletions in correlation to the disease status.METHODS: We analyzed the complete cog-PAI of 174 representative Helicobacter pylori (H pylori) clinical isolates obtained from patients with duodenal ulcer, gastric ulcer, gastric cancer, and non-ulcer dyspepsia using eight different oligonucleotide primers viz cagA1, cagA2, cagAP1, cagAP2, cagE, cagT, LEC-1, LEC-2 spanning five different loci of the whole cog-PAL by polymerase chain reaction (PCR).RESULTS: The complete screening of the genes comprising the cog-PAL showed that larger proportions of subjects with gastric ulcer (97.8%) inhabited strains with complete cog-PAL, followed by gastric cancer (85.7%), non-ulcer dyspepsia (7.1%), and duodenal ulcer (6.9%), significant differences were found in the percentage distribution of the genes in all the clinical groups studied. It was found that strains with complete cog-PAL were able to cause severe histological damage than with the partially deleted ones.CONCLUSION: The cog-PAI is a strong virulent marker in the disease pathogenesis as it is shown that a large number of those infected with strain with complete cag-PAI had one or the other of the irreversible gastric pathologies and interestingly 18.5% of them developed gastric carcinoma. The presence of an intact cag-PAI correlates with the development of more severe pathology, and such strains were found more frequently in patients with severe gastroduodenal disease. Partial deletions of the cog-PAI appear to be sufficient to render the organism less pathogenic. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.