USE OF N-TERMINAL MODIFIED POLY(L-LYSINE) ANTIBODY CONJUGATE AS A CARRIER FOR TARGETED GENE DELIVERY IN MOUSE LUNG ENDOTHELIAL-CELLS
USE OF N-TERMINAL MODIFIED POLY(L-LYSINE) ANTIBODY CONJUGATE AS A CARRIER FOR TARGETED GENE DELIVERY IN MOUSE LUNG ENDOTHELIAL-CELLS
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DOI：
10.1021/bc00016a011
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发表时间：
1992-07-01
影响因子：
4.7
通讯作者：
HUANG, L
中科院分区：
文献类型：
--
作者：
TRUBETSKOY, VS;TORCHILIN, VP;HUANG, L
基于n端修饰的聚l -赖氨酸（NPLL）抗体偶联物，设计了一种易于与质粒DNA形成复合物的DNA靶向传递和表达系统。利用结合NPLL的抗细胞表面血栓调节蛋白的单克隆抗体，在体外将外源质粒DNA靶向递送到表达抗原的小鼠肺内皮细胞系，并在体内靶向递送到小鼠肺。在这两种情况下，大量的DNA都可以特异性地与目标细胞或组织结合。在处理后的小鼠肺内皮细胞中观察到特异性基因表达。
A DNA targeted delivery and expression system has been designed based on an N-terminal modified poly(L-lysine) (NPLL)-antibody conjugate, which readily forms a complex with plasmid DNA. Monoclonal antibodies against the cell-surface thrombomodulin conjugated with NPLL were used for targeted delivery of foreign plasmid DNA to an antigen-expressing mouse lung endothelial cell line in vitro and to mouse lungs in vivo. In both cases significant amounts of DNA can be specifically bound to the target cells or tissues. Specific gene expression was observed in the treated mouse lung endothelial cells.