Biosynthesis of respiratory-tract mucins. A comparison of canine epithelial goblet-cell and submucosal-gland secretions.
Biosynthesis of respiratory-tract mucins. A comparison of canine epithelial goblet-cell and submucosal-gland secretions.
复制标题
呼吸道粘蛋白的生物合成。
作者：
G. H. Stahl;Daniel B. Ellis
1.采用改良的犬气管器官培养系统，研究了上皮杯状细胞和粘膜下腺体粘液分泌的差异。2.通过从表面上皮去除杯状细胞、纤毛细胞和基底细胞来制备裸露的外植体，留下完整的基底膜和有活力的粘膜下层。3.裸露的外植体积极纳入放射性前体分泌的大分子培养时，在培养基199含有标签。4. Bio-GelA-150 m层析和1%琼脂糖凝胶电泳表明上皮杯状细胞分泌物比粘膜下腺体分泌物硫酸化程度相对较高。5.腺体结构被证明对拟副交感神经药物有反应。
1. A modified canine tracheal organ culture system was used to investigate differences between mucous secretions of epithelial goblet cells and the submucosal glands. 2. Denuded explants were prepared by removing goblet, ciliated and basal cells from the surface epithelium leaving an intact basement membrane and viable submucosa. 3. Denuded explants actively incorporated radioactive precursors into secreted macromolecules when cultured in medium 199 containing label. 4. Chromatography on Bio-Gel A-150m and electrophoresis on 1% agarose gels indicated that epithelial goblet cell secretions were relatively more sulphated than submucosal glandular secretions. 5. The glandular structures were shown to respond to a parasympathomimetic agent.