Increased Cell Bond Tension Governs Cell Sorting at the Drosophila Anteroposterior Compartment Boundary
Increased Cell Bond Tension Governs Cell Sorting at the Drosophila Anteroposterior Compartment Boundary
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DOI：
10.1016/j.cub.2009.10.021
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发表时间：
2009-12-01
期刊：
影响因子：
9.2
通讯作者：
Dahmann, Christian
中科院分区：
文献类型：
--
作者：
Landsberg, Katharina P.;Farhadifar, Reza;Dahmann, Christian
将增殖组织细分为区室是动物发育的进化保守策略[1-6]。跨越区室之间边界的信号可导致组织生长和组织图案化的分泌蛋白的局部表达[1-6]。车厢之间的尖锐和笔直的接口对于稳定这些组织者的位置至关重要，因此对于精确执行身体计划至关重要。维持增殖组织中的边界需要机制来对抗由细胞分裂引起的细胞重排;然而，这种机制的性质仍不清楚。在这里，我们定量分析了细胞形态和响应激光消融的细胞债券在附近的前后室边界发展果蝇翅膀。我们发现，机械张力增加约2.5倍，细胞债券沿着这间室边界相比，其余的组织。在存在Y-27632的情况下，细胞键张力降低[7]，Y-27632是Rho激酶的抑制剂，其主要效应物是肌球蛋白II [8]。使用顶点模型[9]的模拟表明，局部细胞键张力增加2.5倍足以引导细胞分裂后的细胞重排，以保持隔室边界。我们的研究结果提供了一个物理机制，其中肌球蛋白II依赖的细胞键张力的局部增加，指导细胞分选室边界。
Subdividing proliferating tissues into compartments is an evolutionarily conserved strategy of animal development [1-6]. Signals across boundaries between compartments can result in local expression of secreted proteins organizing growth and patterning of tissues [1-6]. Sharp and straight interfaces between compartments are crucial for stabilizing the position of such organizers and therefore for precise implementation of body plans. Maintaining boundaries in proliferating tissues requires mechanisms to counteract cell rearrangements caused by cell division; however, the nature of such mechanisms remains unclear. Here we quantitatively analyzed cell morphology and the response to the laser ablation of cell bonds in the vicinity of the anteroposterior compartment boundary in developing Drosophila wings. We found that mechanical tension is approximately 2.5-fold increased on cell bonds along this compartment boundary as compared to the remaining tissue. Cell bond tension is decreased in the presence of Y-27632 [7], an inhibitor of Rho-kinase whose main effector is Myosin II [8]. Simulations using a vertex model [9] demonstrate that a 2.5-fold increase in local cell bond tension suffices to guide the rearrangement of cells after cell division to maintain compartment boundaries. Our results provide a physical mechanism in which the local increase in Myosin II-dependent cell bond tension directs cell sorting at compartment boundaries.