Long-term maintenance of a microfluidic 3D human liver sinusoid.
Long-term maintenance of a microfluidic 3D human liver sinusoid.
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长期维持微流体3D人肝正弦曲线。
DOI：
10.1002/bit.25700
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发表时间：
2016-01
影响因子：
3.8
通讯作者：
Usta OB
中科院分区：
文献类型：
--
作者：
Prodanov L;Jindal R;Bale SS;Hegde M;McCarty WJ;Golberg I;Bhushan A;Yarmush ML;Usta OB
开发长期人类器官型肝脏芯片模型以成功预测毒性反应是NIH/DARPA在动物中复制和取代慢性和急性药物测试的倡议的最重要和最紧迫的目标之一。为此，我们开发了一种微流控芯片，由两个微流控室由多孔膜分隔。本次交流的目的是证明仅使用人类细胞在28天内重现肝窦状隙芯片。使用一步一步的方法来建立一个3D微组织芯片上，我们证明了器官型的体外模型，重组肝窦微结构可以成功地维持一段时间的28天。此外，与静态培养物相比，在流动条件下观察到更高的白蛋白合成（合成）、尿素排泄（解毒）。这种人类肝脏芯片应该在药物相关研究中进一步评估。
The development of long-term human organotypic liver-on-a-chip models for successful prediction of toxic response is one of the most important and urgent goals of the NIH/DARPA’s initiative to replicate and replace chronic and acute drug testing in animals. For this purpose we developed a microfluidic chip that consists of two microfluidic chambers separated by a porous membrane. The aim of this communication is to demonstrate the recapitulation of a liver sinusoid-on-a-chip using human cells only for a period of 28 days. Using a step-by-step method for building a 3D microtissue on-a-chip, we demonstrate that an organotypic in vitro model that reassembles the liver sinusoid microarchitecture can be maintained successfully for a period of 28 days. In addition, higher albumin synthesis (synthetic), urea excretion (detoxification) was observed under flow compared to static cultures. This human liver-on-a-chip should be further evaluated in drug-related studies.