Calcitonin gene-related peptide increases following axotomy of trochlear motoneurons.
Calcitonin gene-related peptide increases following axotomy of trochlear motoneurons.
复制标题
滑车运动神经元轴切术后降钙素基因相关肽增加。
DOI：
10.1006/exnr.1993.1148
复制
发表时间：
1993
影响因子：
5.3
通讯作者：
Murphy,EH
中科院分区：
文献类型：
--
作者：
Wang,XH;Iannuzzelli,PG;Murphy,EH
神经肽长期以来被认为是神经递质或神经调节剂，但它们也可能参与各种调节和营养神经元功能。在本研究中，我们测定了腋切开术对成年猫滑车运动神经元（TMNs）中降钙素基因相关肽（CGRP）水平的影响。可检测到CGRP免疫反应性的神经元数量及其CGRP免疫反应性强度在截轴后1周显著增加，逐渐恢复到正常水平，但仍明显高于截轴后12周的正常水平，此时上斜肌的轴突再生和功能再神经支配应该完成。我们观察到，切开后颞叶神经网络中的CGRP水平增加，表明这种肽在运动神经元对损伤的反应和再生中起作用。此外，由于许多被切开的tmn死亡，我们认为即使在再生完成后，维持高CGRP水平可能反映了存活的tmn的负荷增加。
Neuropeptides have long been considered to act as neurotransmitters or neuromodulators, but they may also contribute to a variety of regulatory and trophic neuronal functions. In the present study, we determined the effects of axotomy on the levels of the neuropeptide calcitonin gene-related peptide (CGRP) in trochlear motoneurons (TMNs) of adult cats. The number of neurons with detectable CGRP immunoreactivity, and the intensity of their CGRP immunoreactivity, increased dramatically 1 week postaxotomy, gradually returned toward normal levels, but remained significantly higher than normal 12 weeks postaxotomy—a time when axonal regeneration and functional reinnervation of the superior oblique muscle should be complete. Our observation that CGRP levels in TMNs increase after axotomy suggests a role for this peptide in the response of motoneurons to injury and in regeneration. In addition, since many axotomized TMNs die, we suggest that the maintenance of high CGRP levels even after regeneration is complete may reflect an increased load on those TMNs that survive.