Axon branching and synaptic bouton phenotypes in GABAergic nonpyramidal cell subtypes
Axon branching and synaptic bouton phenotypes in GABAergic nonpyramidal cell subtypes
复制标题
DOI：
10.1523/jneurosci.4814-03.2004
复制
发表时间：
2004-03-24
影响因子：
5.3
通讯作者：
Kawaguchi, Y
中科院分区：
文献类型：
--
作者：
Karube, F;Kubota, Y;Kawaguchi, Y
GABA能非锥体细胞，皮质中间神经元，包括不同的轴突领域和目标选择性的异质亚型。不同的神经支配模式是如何产生的，以及这些空间复杂但突触特异性的布线是如何实现的，还有待研究。在这里，我们问一个特定的细胞类型是否服从一个特定的分支和bouton安排原则或不同于其他人只有在平均形态测量值的形态模板共同nonpyramidal细胞。为了这个目的，我们细分非锥体细胞在每个生理类的胞体，树突和轴突的定量参数和特征轴突分支和终扣分布模式定量。每个亚型表现出一组特征的垂直和水平的布顿蔓延周围的胞体。每个参数，如分支角度，节间或interbouton间隔，遵循自己的特征分布模式，无论亚型，这表明非锥体细胞有共同的机制，形成轴突分支模式和bouton安排。节间和节间间隔分布的指数拟合表明，它们明显的随机发生。衰减常数的拟合指数各不相同的nonpyramidal细胞，但每个亚型表示一组特定的interbouton和节间间隔平均值。神经支配场的形状和局部轴突表型的独特组合表明，不同GABA能亚型的层状和柱状整合特性以及抑制靶点的亚型特异性密度存在显著的功能差异。
GABAergic nonpyramidal cells, cortical interneurons, consist of heterogeneous subtypes differing in their axonal field and target selectivity. It remains to be investigated how the diverse innervation patterns are generated and how these spatially complicated, but synaptically specific wirings are achieved. Here, we asked whether a particular cell type obeys a specific branching and bouton arrangement principle or differs from others only in average morphometric values of the morphological template common to nonpyramidal cells. For this purpose, we subclassified nonpyramidal cells within each physiological class by quantitative parameters of somata, dendrites, and axons and characterized axon branching and bouton distribution patterns quantitatively. Each subtype showed a characteristic set of vertical and horizontal bouton spreads around the somata. Each parameter, such as branching angles, internode or interbouton intervals, followed its own characteristic distribution pattern irrespective of subtypes, suggesting that nonpyramidal cells have the common mechanism for formation of the axon branching pattern and bouton arrangement. Fitting of internode and interbouton interval distributions to the exponential indicated their apparent random occurrence. Decay constants of the fitted exponentials varied among nonpyramidal cells, but each subtype expressed a particular set of interbouton and internode interval averages. The distinctive combination of innervation field shape and local axon phenotypes suggests a marked functional difference in the laminar and columnar integration properties of different GABAergic subtypes, as well as the subtype-specific density of inhibited targets.