Evaluation of polymer scaffolds to be used in a composite injectable system for intervertebral disc tissue engineering
Evaluation of polymer scaffolds to be used in a composite injectable system for intervertebral disc tissue engineering
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DOI：
10.1002/jbm.a.30250
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发表时间：
2005-07-01
影响因子：
4.9
通讯作者：
Burg, KJL
中科院分区：
文献类型：
--
作者：
Brown, RQ;Mount, A;Burg, KJL
将成年猪髓核细胞接种于明胶、脱钙骨基质和聚乳酸支架上进行体外培养。使用生化分析、组织学和实时定量逆转录酶-聚合酶链反应分析了细胞对支架的反应行为。扫描电子显微镜显示支架的表面纹理有明显差异。髓核细胞在明胶和DBM支架上附着并呈现细长的成纤维细胞样形态。在明胶和DBM上培养的细胞具有代谢活性，并表达I型和11型胶原和聚集蛋白聚糖。在含有聚乳酸支架的培养管中观察到具有圆形形态的分离的细胞聚集体。表面化学和纹理可能在引起细胞对支架材料的反应行为差异中起作用。使用明胶和去矿化骨支架观察到了有希望的结果，但在这些支架上培养的细胞的行为需要进一步表征。这一初步研究将用于指导未来的工作，涉及开发这种复合可注射组织工程系统。(c)2005 Wiley Periodicals，Inc. J Biomed Mater Res 74A：32-39，2005.
Adult porcine nucleus pulposus cells were seeded onto gelatin, demineralized bone matrix (DBM), and polylactide scaffolds and cultured in vitro. Cellular behavior in response to the scaffolds was analyzed using biochemical assays, histology, and real-time quantitative reverse transcriptase-polymerase chain reaction. Scanning electron microscopy showed pronounced differences in surface texture of the scaffolds. Nucleus pulposus cells attached and assumed an elongated fibroblast-like morphology on the gelatin and DBM scaffolds. The cells cultured on the gelatin and DBM were metabolically active and expressed types I and 11 collagen and aggrecan. Detached cellular aggregates with a rounded morphology were noted in the culture tubes containing the polylactide scaffolds. Both surface chemistry and texture likely had a role in causing differences in cellular behavior in response to scaffold material. Promising results were observed using the gelatin and dernineralized bone scaffolds, but the behavior of cells cultured on these scaffolds will need to be characterized further. This initial research will be used to direct future work involved in developing this composite injectable tissue engineering system. (c) 2005 Wiley Periodicals, Inc. J Biomed Mater Res 74A: 32-39, 2005.