Heparin-induced release of DNA template restrictions in human sperm zinc-depleted nuclei.
Heparin-induced release of DNA template restrictions in human sperm zinc-depleted nuclei.
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肝素诱导人类精子缺锌细胞核中 DNA 模板限制的释放。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
R. Reyes
中科院分区：
文献类型：
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作者：
N. Delgado;V. Magdaleno;H. Merchant;A. Rosado;R. Reyes
肝素加入到人类精子锌耗尽的细胞核释放DNA模板的限制。对锌耗竭的精子进行（3 H-甲基）测定，观察到胸苷掺入（3 H-甲基胸苷27，500 +/-1，248 dpm）。在10 mg/ml大豆胰蛋白酶抑制剂存在下孵育的精子细胞对精子核肿胀或DNA模板限制的释放无影响。这一过程与肝素诱导的细胞核肿胀平行，表明肿胀的细胞核可能是DNA模板的来源。这一点得到了放射自显影研究的证实，因为所有通过形态学标准判断细胞核肿胀的精细胞也出现了标记。由于不需要ATP生成系统或外源DNA聚合酶，锌在人精子的生理活动中起着重要的调控作用。
The addition of heparin to human sperm zinc-depleted nuclei releases DNA template restrictions. Spermatozoa depleted of zinc were assayed for (3H-methyl), thymidine incorporation was observed (27,500 +/- 1,248 dpm of 3H methyl-thymidine). Sperm cells incubated in the presence of 10 mg/ml of soybean trypsin inhibitor shows no effect in sperm nuclear swelling or in the release of DNA template restrictions. This process runs in a parallel fashion to the nuclear swelling induced by heparin, suggesting that swollen nuclei might be the source of DNA template. This was confirmed by autoradiographic studies, since all the sperm cells whose nuclei were judged swollen by morphological criteria also appeared labeled. The fact that there was no need for ATP generating system or of exogenous DNA polymerase emphasized the control role that zinc plays in the physiology of the human spermatozoa.