Family environments and leukocyte transcriptome indicators of a proinflammatory phenotype in children and parents.
Family environments and leukocyte transcriptome indicators of a proinflammatory phenotype in children and parents.
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儿童和父母促炎表型的家庭环境和白细胞转录组指标。
DOI：
10.1017/s0954579417000591
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发表时间：
2018
影响因子：
3.3
通讯作者：
Cole,StevenW
中科院分区：
文献类型：
--
作者：
Robles,TheodoreF;Repetti,RenaL;Reynolds,BridgetM;Chung,PaulJ;Arevalo,JesusaMG;Cole,StevenW
家庭中的高冲突和低温暖可能有助于免疫细胞发展出一种倾向，即对抑制信号不敏感的夸大炎症反应的威胁。我们检测了家庭环境与携带调节炎症的转录因子反应元件的基因表达之间的关系：核因子κ B（NF-κB）和糖皮质激素受体。整体样本（47个家庭）完成了访谈，问卷调查，8周的日常日记评估的冲突和温暖，这是用来创建复合家庭冲突和温暖的分数。这些日记评估了上呼吸道感染（URI）症状，并对URI发作进行了临床验证。从42名儿童（8-13岁）和73名父母的子样本提供的全血样本中提取白细胞RNA。在儿童中，较高的冲突和较低的温暖与携带促炎转录因子NF-κB反应元件的基因的较高表达和较严重的URI症状有关。在父母中，较高的冲突和较低的温暖也与较高的NF-κ B相关基因表达有关。单核细胞和树突状细胞是样本中差异基因表达的主要细胞来源。与现有的概念框架一致，压力的家庭环境与循环白细胞转录组水平的促炎表型相关。
High conflict and low warmth in families may contribute to immune cells developing a tendency to respond to threats with exaggerated inflammation that is insensitive to inhibitory signaling. We tested associations between family environments and expression of genes bearing response elements for transcription factors that regulate inflammation: nuclear factor kappa B (NF-κB) and glucocorticoid receptor. The overall sample (47 families) completed interviews, questionnaires, and 8-week daily diary assessments of conflict and warmth, which were used to create composite family conflict and warmth scores. The diaries assessed upper respiratory infection (URI) symptoms, and URI episodes were clinically verified. Leukocyte RNA was extracted from whole blood samples provided by a subsample of 42 children (8–13 years of age) and 73 parents. In children, higher conflict and lower warmth were related to greater expression of genes bearing response elements for the proinflammatory transcription factor NF-κB, and more severe URI symptoms. In parents, higher conflict and lower warmth were also related to greater NF-κB–associated gene expression. Monocytes and dendritic cells were implicated as primary cellular sources of differential gene expression in the sample. Consistent with existing conceptual frameworks, stressful family environments were related to a proinflammatory phenotype at the level of the circulating leukocyte transcriptome.