Transverse imaging and simulation of dsDNA electrophoresis in microfabricated glass channels.
Transverse imaging and simulation of dsDNA electrophoresis in microfabricated glass channels.
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微加工玻璃通道中 dsDNA 电泳的横向成像和模拟。
DOI：
10.1002/elps.200800204
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发表时间：
2008
期刊：
影响因子：
2.9
通讯作者：
Burns,MarkA
中科院分区：
文献类型：
--
作者：
Zeitoun,RamseyI;Chen,Zheng;Burns,MarkA
We have observed the non‐uniform distribution of DNA molecules during PAGE in a microfabricated system. Confocal laser scanning microscopy was used to visualize fluorescently labeled DNA during electrophoretic migration. The distribution of double‐stranded DNA larger than 100 bp is observed to transition from a center‐biased motion on the transverse plane 1 cm downstream from injection to an edge‐biased motion 2 cm downstream. Although this distribution increased with increasing dsDNA size in a cross‐linked gel, no similar distribution was found with the same dsDNA molecules in a linear polyacrylamide solution (6%). Simulations of DNA distribution in gels suggest that DNA distribution non‐uniformities may be caused by biased electrophoretic migration resulting from motion in an inhomogeneous gel system.
