MicroRNA-351 regulates TMEM59 (DCF1) expression and mediates neural stem cell morphogenesis
MicroRNA-351 regulates TMEM59 (DCF1) expression and mediates neural stem cell morphogenesis
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MicroRNA-351 调节 TMEM59 (DCF1) 表达并介导神经干细胞形态发生
DOI：
10.4161/rna.19100
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发表时间：
2012-03-01
期刊：
影响因子：
4.1
通讯作者：
Wen, Tieqiao
中科院分区：
文献类型：
--
作者：
Li, Xiao;Feng, Ruili;Wen, Tieqiao
microRNA（miRNA）是一类在细胞发育、分化和凋亡过程中起重要作用的非编码小分子RNA。最近的研究表明，跨膜蛋白59（TMEM 59）影响神经干细胞（NSC）分化和β淀粉样前体蛋白（APP）糖基化。在这项研究中，我们预测microRNA-351（miR-351）可以靶向TMEM 59，并证明miR-351在不同细胞类型中负调控TMEM 59的表达。此外，我们发现miR-351过表达可导致小鼠NSC细胞系C17. 2的形态学改变，表明miR-351在分化调节中的重要作用。我们的研究主要集中在miR-351在神经系统中的功能以及microRNA对TMEM 59的转录后调控。这些原始发现为未来深入研究miR-351和TMEM 59在神经系统发育中的功能提供了有希望的基础。
MicroRNAs (miRNA) are small noncoding RNAs that play important roles in cell development, differentiation and apoptosis. Recent studies showed that transmembrane protein 59 (TMEM59) affects neural stem cell (NSC) differentiation and beta-amyloid precursor protein (APP) glycosylation. In this study, we predicted that microRNA-351 (miR-351) could target TMEM59 and demonstrated that miR-351 negatively regulates TMEM59 expression in different cell types. Moreover, we found that miR-351 overexpression could lead to morphological change in the mouse NSC cell line C17.2, indicating an important role of miR-351 in the regulation of differentiation. Our investigations focused on the functions of miR-351 in the nervous system and the posttranscriptional regulation of TMEM59 by microRNA. These original findings provide promising grounds for future in-depth research into the functions of miR-351 and TMEM59 in nervous system development.