Rapid identification of fruit length loci in cucumber (Cucumis sativus L.) using next-generation sequencing (NGS)-based QTL analysis.
Rapid identification of fruit length loci in cucumber (Cucumis sativus L.) using next-generation sequencing (NGS)-based QTL analysis.
复制标题
使用基于下一代测序 (NGS) 的 QTL 分析快速鉴定黄瓜 (Cucumis sativus L.) 的果实长度基因座
DOI：
10.1038/srep27496
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发表时间：
2016-06-07
影响因子：
4.6
通讯作者：
Chen JF
中科院分区：
文献类型：
--
作者：
Wei QZ;Fu WY;Wang YZ;Qin XD;Wang J;Li J;Lou QF;Chen JF
黄瓜（Cucumis sativus L.）在果实大小和形状上表现出广泛的变化。果长是一个重要的农艺性状和驯化性状，受数量性状基因座（QTL）控制。尽管如此，决定黄瓜果实长度的潜在分子和遗传机制仍不清楚。QTL-seq是一种有效的QTL鉴定策略，它利用了混合分离分析（BSA）和下一代测序（NGS）。本研究对黄瓜果实长度进行了QTL定位和QTL-seq分析。QTL定位共检测到8个影响未成熟果长和成熟果长的QTL。检测到一个主效QTL fl3.2，最大解释表型变异的38.87%。全基因组范围内比较两个DNA块之间的SNP谱，确定了6个子房长度的QTL。对子房长度有主效作用的QTL为ovl3.1和ovl3.2，SNP指数分别为0.80和0.74（P0.01）。对定位于共有QTL FL3.2的果实大小相关同源基因进行了定量RT-PCR。四个候选基因在长果基因型中表现出较高的表达水平。本研究结果表明了QTL-seq方法在快速QTL检测中的强大功能，为果长相关基因的精细定位和候选基因的鉴定提供了可靠的QTL区域。
The cucumber (Cucumis sativus L.) exhibits extensive variations in fruit size and shape. Fruit length is an important agronomic and domesticated trait controlled by quantitative trait loci (QTLs). Nonetheless, the underlying molecular and genetic mechanisms that determine cucumber fruit length remain unclear. QTL-seq is an efficient strategy for QTL identification that takes advantage of bulked-segregant analysis (BSA) and next-generation sequencing (NGS). In the present study, we conducted QTL mapping and QTL-seq of cucumber fruit length. QTL mapping identified 8 QTLs for immature and mature fruit length. A major-effect QTL fl3.2, which explained a maximum of 38.87% of the phenotypic variation, was detected. A genome-wide comparison of SNP profiles between two DNA bulks identified 6 QTLs for ovary length. QTLs ovl3.1 and ovl3.2 both had major effects on ovary length with a △ (SNP-index) of 0.80 (P 0.01) and 0.74 (P 0.01), respectively. Quantitative RT-PCR of fruit size-related homologous genes localized in the consensus QTL FL3.2 was conducted. Four candidate genes exhibited increased expression levels in long fruit genotypes. Our results demonstrated the power of the QTL-seq method in rapid QTL detection and provided reliable QTL regions for fine mapping of fruit length-related loci and for identifying candidate genes.