Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the arabidopsis flowering time gene CONSTANS
Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the arabidopsis flowering time gene CONSTANS
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DOI：
10.1105/tpc.12.12.2473
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发表时间：
2000-12-01
期刊：
影响因子：
11.6
通讯作者：
Sasaki, T
中科院分区：
文献类型：
--
作者：
Yano, M;Katayose, Y;Sasaki, T
利用图位克隆技术，对一个控制光周期反应的主效数量性状位点Hdl进行了定位。使用1505个分离子的高分辨率作图使我们能够将类似于12 kb的基因组区域定义为Hdl的候选者。进一步的分析显示，Hdl QTL对应于拟南芥中CONSTANS的同源基因。测序分析显示，光周期敏感性1（se 1）突变体HS 66的第一个外显子中的43-bp的缺失和突变体HS 110的内含子中的433-bp的插入。通过对两个se 1突变体HS 66和HS 110的分析，确定Se 1与Hdl QTL是等位的。遗传互补分析证实了候选基因的功能。光周期长度的变化对高密度脂蛋白mRNA的表达量影响不大。我们认为，高密度脂蛋白的功能在短日照条件下，在长日照条件下，在促进抽穗和抑制。
A major quantitative trait locus (QTL) controlling response to photoperiod, Hdl, was identified by means of a map-based cloning strategy. High-resolution mapping using 1505 segregants enabled us to define a genomic region of similar to 12 kb as a candidate for Hdl. Further analysis revealed that the Hdl QTL corresponds to a gene that is a homolog of CONSTANS in Arabidopsis. Sequencing analysis revealed a 43-bp deletion in the first exon of the photoperiod sensitivity 1 (se1) mutant HS66 and a 433-bp insertion in the intron in mutant HS110. Se1 is allelic to the Hdl QTL, as determined by analysis of two se1 mutants, HS66 and HS110. Genetic complementation analysis proved the function of the candidate gene. The amount of Hdl mRNA was not greatly affected by a change in length of the photoperiod. We suggest that Hdl functions in the promotion of heading under short-day conditions and in inhibition under long-day conditions.