Species, sex, and individual identification of Japanese serow (Capricornis crispus) and sika deer (Cervus nippon) in sympatric region based on the fecal DNA samples
Species, sex, and individual identification of Japanese serow (Capricornis crispus) and sika deer (Cervus nippon) in sympatric region based on the fecal DNA samples
复制标题
基于粪便DNA样本对同域日本羚羊（Capricornis Cripus）和梅花鹿（Cervus nippon）的种类、性别和个体进行鉴定
DOI：
10.1007/s12686-016-0665-1
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
and Yamashiro T.
中科院分区：
文献类型：
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作者：
Yamashiro A.;Kaneshiro Y.;Kawaguchi Y.;and Yamashiro T.
我们描述了非侵入性的分子技术的物种，性别和个人识别使用粪便的同域物种，日本serow（Capricornis crispus）和西卡鹿（Cervus nippon），在日本。从19个微卫星筛选52个组织样品的两个物种，我们选择了14个和10个高度多态性位点的日本serow和西卡鹿，分别，其中5个位点可以在物种之间扩增。首先，使用两个跨物种可重复的基因座和性别鉴定的基础上的部分釉原蛋白基因序列的194个粪便样本从东四国，日本进行了物种鉴定。PCR扩增65个粪便样品的新的和相对新的粪粒确定了35个日本serow和30个西卡鹿。在日本serow，14个个体（7男7女）被确定使用11的14个微卫星。在西卡鹿，19个个体（11名男性和8名女性）被确定使用9的10个微卫星。这种利用粪便样本的非侵入性遗传学技术对研究同域分布的日本长羚和西卡的分布、基因流、性别比和种群规模是有用的。
We describe non-invasive molecular techniques for species, sex, and individual identification using fecal of the sympatric species, Japanese serow (Capricornis crispus) and sika deer (Cervus nippon), in Japan. From screening 19 microsatellites using 52 tissue samples of the two species, we selected 14 and 10 highly polymorphic loci for Japanese serow and sika deer, respectively, of which five loci could be amplified across the species. First, species identification using two cross-species-amplifiable loci and sex identification based on a partial amelogenin gene sequence were conducted on 194 fecal samples from Eastern Shikoku, Japan. PCR amplification of 65 fecal samples for new and relatively new fecal pellets identified 35 Japanese serow and 30 sika deer. In Japanese serow, 14 individuals (7 males and 7 females) were identified using 11 of 14 microsatellites. In sika deer, 19 individuals (11 males and 8 females) were identified using 9 of 10 microsatellites. This non-invasive genetic technique using fecal samples should be useful to study the distribution, gene flow, sex ratio, and population size of sympatric Japanese serow and sika deer.