Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats
Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats
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DOI：
10.1016/j.cbi.2008.12.012
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发表时间：
2009-05-15
影响因子：
5.1
通讯作者：
Prince, P. Stanely Mainzen
中科院分区：
文献类型：
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作者：
Priscilla, D. Hansi;Prince, P. Stanely Mainzen
目前，全球范围内对鉴定具有药理作用且副作用低或无副作用的抗氧化剂化合物的兴趣日益浓厚，可用于预防医学。本研究旨在评估没食子酸对异丙肾上腺素 (ISO) 诱导的雄性 Wistar 大鼠心肌标志酶、肌钙蛋白-T、LDH-同工酶模式、脂质过氧化产物和抗氧化状态的保护作用。雄性白化 Wistar 大鼠每天用没食子酸（15 mg/kg）预处理，持续 10 天。治疗期结束后，给大鼠皮下注射ISO（100mg/kg），间隔24小时，持续2天。 ISO 诱导的心肌损伤表现为血清中肌酸激酶、肌酸激酶-MB、天冬氨酸转氨酶、丙氨酸转氨酶和乳酸脱氢酶等标记酶活性的升高以及血清中肌钙蛋白-T 水平的升高。在 ISO 诱导的大鼠血清中也观察到 LDH 同工酶带（LDH-1 和 LDH-2）增加。除这些诊断标志物外，血浆和心脏中的脂质过氧化产物水平显着升高（P < 0.05），心脏中的酶抗氧化剂（如超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽-S-转移酶）和非酶抗氧化剂（如谷胱甘肽、 ISO 诱导的大鼠血浆和心脏中的维生素 C 和 E 显着降低（P < 0.05）。 ISO处理的大鼠血浆中尿酸水平显着升高（P<0.05）。没食子酸预处理对所有研究的生化参数均显示出显着的保护作用。没食子酸预处理的心肌梗塞心脏的组织病理学结果证实了本研究的生化结果。因此，没食子酸可以保护心肌免受异丙肾上腺素诱导的氧化应激。 (c) 2008 Elsevier Ireland Ltd. 保留所有权利。
Currently there has been an increased interest globally to identify antioxidant compounds that are pharmacologically potent and have low or no side effects for use in preventive medicine. This study was designed to evaluate the protective effect of gallic acid on cardiac marker enzymes, troponin-T, LDH-isoenzyme pattern, lipid peroxidation products and antioxidant status in isoproterenol (ISO)-induced myocardial infarction in male Wistar rats. Male albino Wistar rats were pretreated with gallic acid (15 mg/kg) daily for a period of 10 days. After the treatment period, ISO (100 mg/kg) was subcutaneously injected to rats at an interval of 24 h for 2 days. ISO-induced myocardial damage was indicated by increased activities of marker enzymes such as creatine kinase, creatine kinase-MB, aspartate transaminase, alanine transaminase and lactate dehydrogenase in serum and the levels of troponin-T in the serum. Increased LDH-isoenzyme bands (LDH-1 and LDH-2) were also observed in serum of ISO-induced rats. In addition to these diagnostic markers, the levels of lipid peroxidation products in plasma and the heart were significantly (P < 0.05) increased and the activities of enzymic antioxidants such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase in the heart and non-enzymic antioxidants such as glutathione, vitamin C and E in plasma and the heart were significantly (P < 0.05) decreased in ISO-induced rats. The level of uric acid in plasma was significantly (P < 0.05) increased in ISO-treated rats. Gallic acid pretreatment showed significant protective effect on all the biochemical parameters studied. Histopathological findings of gallic acid pretreated myocardial infarcted heart confirmed the biochemical findings of this study. Thus, gallic acid protects the myocardium against isoproterenol-induced oxidative stress. (c) 2008 Elsevier Ireland Ltd. All rights reserved.