Generation of reactive oxygen species in thylakoids from senescing flag leaves of the barley varieties Lomerit and Carina
Generation of reactive oxygen species in thylakoids from senescing flag leaves of the barley varieties Lomerit and Carina
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DOI：
10.1007/s00425-015-2274-8
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发表时间：
2015-06-01
期刊：
影响因子：
4.3
通讯作者：
Krupinska, Karin
中科院分区：
文献类型：
--
作者：
Krieger-Liszkay, Anja;Troesch, Mirl;Krupinska, Karin
在衰老过程中，类囊体中活性氧的产生增加。在两个大麦品种中，超氧阴离子的产生没有差异，而单线态氧的产生只在一个品种中增加。在衰老过程中，叶绿素含量下降，光合电子传递抑制所示的大麦品种Lomerit和Carina在田间生长的剑叶。利用自旋捕获电子顺磁共振（EPR）技术研究了类囊体膜衰老过程中活性氧的产生。EPR测量与特定的自旋陷阱进行区分单线态氧一方面和活性氧中间体的另一方面。结果表明，在衰老过程中，活性氧中间产物的产生在两个品种增加。单线态氧只在品种cv。洛美利，而它保持恒定在低水平的品种cv。卡瑞娜在光系统II和细胞色素b6f复合物的抑制剂的存在下进行的测量表明，在衰老的叶片中，光系统I受体侧的氧的减少是主要的，但不是超氧阴离子的唯一来源。本研究表明，在衰老过程中，个别活性氧的产生不同的大麦品种。
During senescence, production of reactive oxygen species increased in thylakoids. In two barley varieties, no difference in superoxide production was observed while singlet oxygen production increased only in one variety.During senescence, chlorophyll content decreased and photosynthetic electron transport was inhibited as shown for flag leaves collected from barley varieties Lomerit and Carina grown in the field. Spin trapping electron paramagnetic resonance (EPR) was used to investigate the production of reactive oxygen species in thylakoid membranes during senescence. EPR measurements were performed with specific spin traps to discriminate between singlet oxygen on one hand and reactive oxygen intermediates on the other hand. The results show that the generation of reactive oxygen intermediates increases in both varieties during senescence. Singlet oxygen increased only in the variety cv. Lomerit while it remained constant at a low level in the variety cv. Carina. Measurements in the presence of inhibitors of photosystem II and of the cytochrome b6f complex revealed that in senescing leaves reduction of oxygen at the acceptor side of photosystem I was the major, but not the only source of superoxide anions. This study shows that during senescence the production of individual reactive oxygen species varies in different barley varieties.