Survivals of mouse oocytes approach 100% after vitrification in 3-fold diluted media and ultra-rapid warming by an IR laser pulse.
Survivals of mouse oocytes approach 100% after vitrification in 3-fold diluted media and ultra-rapid warming by an IR laser pulse.
复制标题
DOI：
10.1016/j.cryobiol.2014.03.005
复制
发表时间：
2014-06
期刊：
影响因子：
2.7
通讯作者：
Mazur P
中科院分区：
文献类型：
--
作者：
Jin B;Kleinhans FW;Mazur P
玻璃化冷冻是冷冻保存动物胚胎和卵母细胞以及其他具有医学、遗传和农业重要性的细胞的最受欢迎的途径。目前的想法是，成功的玻璃化冷冻需要细胞悬浮在高浓度的保护性溶质中，并被高浓度的保护性溶质渗透，并且以非常高的速度冷却到-100 °C以下。我们在这里报告，这些信念都不适用于小鼠卵母细胞。相反，我们发现，如果小鼠卵母细胞悬浮在玻璃化前产生相当大的渗透脱水的培养基中，随后通过激光脉冲以超高速率（10，000，000 °C/min）加热，即使冷却得相当慢，并且培养基中的溶质浓度仅为标准的1/3，也几乎100%存活。
Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that they be cooled at very high rates to below − 100°C. We report here that neither of these beliefs holds for mouse oocytes. Rather, we find that if mouse oocytes are suspended in media that produce considerable osmotic dehydration before vitrification and are subsequently warmed at ultra high rates (10,000,000°C/min) achieved by a laser pulse, nearly 100% will survive even when cooled rather slowly and when the concentration of solutes in the medium is only 1/3rd of standard.