In vitro evaluation of new biocompatible coatings for solid-phase microextraction: Implications for drug analysis and in vivo sampling applications
In vitro evaluation of new biocompatible coatings for solid-phase microextraction: Implications for drug analysis and in vivo sampling applications
复制标题
DOI：
10.1016/j.aca.2009.02.049
复制
发表时间：
2009-04-13
影响因子：
6.2
通讯作者：
Pawliszyn, Janusz
中科院分区：
文献类型：
--
作者：
Vuckovic, Dajana;Shirey, Robert;Pawliszyn, Janusz
最近开发了一种适用于液相色谱应用的新型固相微萃取（SPME）涂层，以解决目前可用涂层的局限性。拟定涂层固定在金属纤维芯上，由专有生物相容性粘合剂和各种类型的涂层二氧化硅（十八烷基、极性包埋和氰基）颗粒的混合物组成。本研究的目的是在体外评估这些新的SPME纤维，以评估其适用于药物分析和在体内SPME应用。检查的主要参数为浸提效率、耐溶剂性、预处理、浸提动力学对涂层厚度的依赖性、残留、线性范围和纤维间重现性。适用时，将拟定涂层的性能与商业Carbowax-TPR（CW-TPR）涂层进行比较。评价纤维对不同类别药物（卡马西平、普萘洛尔）的提取。伪麻黄碱、雷尼替丁和地西泮）。采用液相色谱-串联质谱法进行分析。结果表明，该纤维对于生物流体的提取表现非常好，不需要样品预处理，并且还可以用于流动血液的体内采样应用。发现45 μ m的涂层厚度是萃取能力和萃取动力学之间的良好折衷。由于这些涂层的高提取效率，可以采用具有非常短的提取时间（2分钟）的预平衡SPME来增加样品通量。纤维间重现性为
A new line of solid-phase microextraction (SPME) coatings suitable for use with liquid chromatography applications was recently developed to address the limitations of the currently available coatings. The proposed coatings were immobilized on the metal fiber core and consisted of a mixture of proprietary biocompatible binder and Various types of coated silica (octadecyl, polar embedded and cyano) particles. The aim of this research was to perform in vitro assessment of these new SPME fibers in order to evaluate their suitability for drug analysis and in vivo SPME applications. The main parameters examined were extraction efficiency, solvent resistance, preconditioning, dependence of extraction kinetics on coating thickness, carryover, linear range and inter-fiber reproducibility. The performance of the proposed coatings was compared against commercial Carbowax-TPR (CW-TPR) coating, when applicable. The fibers were evaluated for the extraction of drugs of different classes (carbamazepine, propranolol. pseudoephedrine, ranitidine and diazepam) from plasma and urine. The analyses were performed using liquid chromatography-tandem mass spectrometry. The results show that the fibers perform very well for the extraction of biological fluids with no sample pre-treatment required and can also be used for in vivo sampling applications of flowing blood. A coating thickness of 45 mu m was found to be a good compromise between extraction capacity and extraction kinetics. Due to the high extraction efficiency of these coatings, pre-equilibrium SPME with very short extraction times (2 min) can be employed to increase sample throughput. Inter-fiber reproducibility was