Polyethylene glycol increases the detection of anti-thyrotropin receptor antibodies by a radioreceptor assay.
Polyethylene glycol increases the detection of anti-thyrotropin receptor antibodies by a radioreceptor assay.
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聚乙二醇通过放射性受体测定增加了抗促甲状腺素受体抗体的检测。
作者：
Y. Watanabe;H. Tada;Y. Hidaka;T. Takano;K. Takeoka;S. Fukata;K. Kuma;N. Amino
促甲状腺激素（TSH）受体（TSHR）的自身抗体是甲状腺自身免疫反应的标志，是Graves病甲状腺毒症的原因（1）。与其他自身抗体不同，TSHR抗体没有直接测定。相反，这些抗体通过其抑制放射性标记TSH与受体结合的能力或TSHR活化的生物测定来检测（2）（3）。最广泛使用的测定是放射受体测定，其使用溶解的猪TSHR，其中抗体被称为TSH结合抑制性免疫球蛋白（TBII）。血清TBII很容易用市售试剂盒检测;然而，5-10%的Graves病患者显示阴性反应，可能与检测不足有关（4）。
在甲状腺刺激物的体外生物测定中，添加聚乙二醇（PEG）可增强患者IgG诱导的cAMP生成，但不会增强TSH、毛喉素或GTPγS诱导的cAMP生成（5），表明增强发生在抗体-受体相互作用步骤。我们最近发现…
Autoantibodies to the thyrotropin (TSH) receptor (TSHR) are the hallmark of the autoimmune response to the thyroid gland and are responsible for thyrotoxicosis in Graves disease (1). Unlike other autoantibodies, there is no direct assay for TSHR antibodies. Instead, these antibodies are detected either by their ability to inhibit binding of radiolabeled TSH to receptors or by a bioassay of TSHR activation (2)(3). The most widely used assay is a radioreceptor assay, which uses solubilized porcine TSHR, in which the antibody is called TSH-binding inhibitory immunoglobulin (TBII). Serum TBII is easily assayed with a commercially available kit; however, 5–10% of patients with Graves disease show a negative reaction perhaps related to inadequacies of the assay (4).
Addition of polyethylene glycol (PEG) enhances cAMP production induced by a patient’s IgG, but not by TSH, forskolin, or GTPγS in an in vitro bioassay for thyroid stimulators (5), suggesting that the enhancement occurs at the step of antibody-receptor interaction. We recently found …
促甲状腺素受体介导的疾病：受体自身免疫的范例。
DOI：
10.1016/s0167-5699(97)01089-x
发表时间：
1997
期刊：
Immunology today
影响因子：
--
作者：
Prabhakar,BS;Fan,JL;Seetharamaiah,GS
通讯作者：
Seetharamaiah,GS
β-肾上腺素受体偶联腺苷酸环化酶的同源脱敏。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Strasser,RH;Lefkowitz,RJ
通讯作者：
Lefkowitz,RJ