One-step PCR to distinguish B virus from related primate alphaherpesviruses.
One-step PCR to distinguish B virus from related primate alphaherpesviruses.
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一步 PCR 区分 B 病毒和相关灵长类 α 疱疹病毒。
DOI：
10.1128/cdli.9.3.716-719.2002
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Eberle,Richard
中科院分区：
文献类型：
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作者：
Hirano,Makoto;Nakamura,Shin;Mitsunaga,Fusako;Okada,Maki;Shirahama,Shuya;Eberle,Richard
通过向PCR混合物中加入甜菜碱，我们先前建立了一种PCR方法来扩增来自恒河猴的B病毒（BV）的糖蛋白G基因的DNA片段。我们发现，来自食蟹猴、猪尾猴和狮尾猕猴的其他BV菌株的DNA也可以作为我们PCR检测的模板。在相同的条件下，用绿色猴的猴病原体8和狒狒的乳头状疱疹病毒2或人类单纯疱疹病毒1型和2型的DNA都没有得到产物。因此，这种PCR方法是有用的区分BV从其他密切相关的灵长类动物α疱疹病毒。
By adding betaine to the PCR mixture, we previously established a PCR method to amplify a DNA segment of the glycoprotein G gene of B virus (BV) derived from a rhesus macaque. We have found that DNA of other BV strains derived from cynomolgus, pigtail, and lion-tailed macaques can also serve as the template in our PCR assay. Under the same conditions no product was obtained with DNA of simian agent 8 of green monkeys andHerpesvirus papio2 of baboons, or the human herpes simplex viruses types 1 and 2. Thus, this PCR method is useful to discriminate BV from other closely related primate alphaherpesviruses.