Biotechnological and protein-engineering implications of ancestral protein resurrection
Biotechnological and protein-engineering implications of ancestral protein resurrection
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DOI：
10.1016/j.sbi.2018.02.007
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发表时间：
2018-08-01
影响因子：
6.8
通讯作者：
Ozkan, S. Banu
中科院分区：
文献类型：
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作者：
Risso, Valeria A.;Sanchez-Ruiz, Jose M.;Ozkan, S. Banu
祖先蛋白质序列的近似值可以从其现代后代的序列中推导出来。由这种重建序列编码的蛋白质可以在实验室中制备并进行实验检查。这些“复活”的祖先蛋白质通常显示出显着的特性，反映了祖先对细胞内和细胞外环境的适应，这些环境不同于现代/现存蛋白质的环境。最近的实验和计算工作已经具体讨论了高稳定性，底物和催化混杂性，构象的灵活性/多样性和改变与其他亚细胞组分的相互作用模式。在这篇综述中，我们讨论了这些显着的特性，以及最近尝试探索其生物技术和蛋白质工程的潜力。
Approximations to the sequences of ancestral proteins can be derived from the sequences of their modern descendants. Proteins encoded by such reconstructed sequences can be prepared in the laboratory and subjected to experimental scrutiny. These 'resurrected' ancestral proteins often display remarkable properties, reflecting ancestral adaptations to intra-cellular and extra-cellular environments that differed from the environments hosting modern/extant proteins. Recent experimental and computational work has specifically discussed high stability, substrate and catalytic promiscuity, conformational flexibility/diversity and altered patterns of interaction with other sub-cellular components. In this review, we discuss these remarkable properties as well as recent attempts to explore their biotechnological and protein engineering potential.