Laser-synthesized oxide-passivated bright Si quantum dots for bioimaging.
Laser-synthesized oxide-passivated bright Si quantum dots for bioimaging.
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DOI：
10.1038/srep24732
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发表时间：
2016-04-22
影响因子：
4.6
通讯作者：
Timoshenko VY
中科院分区：
文献类型：
--
作者：
Gongalsky MB;Osminkina LA;Pereira A;Manankov AA;Fedorenko AA;Vasiliev AN;Solovyev VV;Kudryavtsev AA;Sentis M;Kabashin AV;Timoshenko VY
晶体硅（Si）纳米颗粒是基于可见光和近红外光谱区域的光致发光（PL）的生物成像的一个非常有前途的对象，但是它们在水悬浮液中的有效PL发射通常在湿化学程序之后观察到，导致残留毒性问题。在这里，我们介绍了超纯激光合成的硅基量子点（QD），这是水分散性，并表现出明亮的激子PL在窗口的相对组织透明度接近800 nm。基于在气态氦中激光烧蚀晶体Si靶，然后在生理盐水中超声辅助分散沉积膜，所提出的方法避免了合成过程中的任何有毒副产物。我们证明了有效的对比度的硅量子点在活细胞中的激子PL。我们还表明，所制备的量子点不会引起任何细胞毒性效应，同时渗透到细胞中，并有效地积累在细胞膜附近和细胞质中。结合实现并行治疗通道的可能性，超纯激光合成的Si纳米结构为癌症治疗诊断应用提供了独特的目标。
Crystalline silicon (Si) nanoparticles present an extremely promising object for bioimaging based on photoluminescence (PL) in the visible and near-infrared spectral regions, but their efficient PL emission in aqueous suspension is typically observed after wet chemistry procedures leading to residual toxicity issues. Here, we introduce ultrapure laser-synthesized Si-based quantum dots (QDs), which are water-dispersible and exhibit bright exciton PL in the window of relative tissue transparency near 800 nm. Based on the laser ablation of crystalline Si targets in gaseous helium, followed by ultrasound-assisted dispersion of the deposited films in physiological saline, the proposed method avoids any toxic by-products during the synthesis. We demonstrate efficient contrast of the Si QDs in living cells by following the exciton PL. We also show that the prepared QDs do not provoke any cytoxicity effects while penetrating into the cells and efficiently accumulating near the cell membrane and in the cytoplasm. Combined with the possibility of enabling parallel therapeutic channels, ultrapure laser-synthesized Si nanostructures present unique object for cancer theranostic applications.