A comparison of the cytoplasmic estrogen receptors of mammary gland from virgin and lactating mice.
A comparison of the cytoplasmic estrogen receptors of mammary gland from virgin and lactating mice.
复制标题
处女小鼠和哺乳期小鼠乳腺细胞质雌激素受体的比较。
作者：
C. Gaubert;S. Biancucci;G. Shyamala
最近的研究表明，处女小鼠乳腺胞浆雌激素受体主要以45种形式沉积在低离子强度蔗糖梯度上。在目前的研究中，我们发现正常和去卵巢的处女鼠和哺乳期小鼠的乳腺胞浆雌激素受体在低离子强度缓冲液中制备的蔗糖梯度上沉积了9s。然而，来自处女小鼠的受体很容易被切割，导致受体出现3-4S形式。然而，如果在胞浆的制备和分析中使用磷酸盐缓冲液或含有钼酸钠的缓冲液，则保留了天然9S雌激素受体的完整性。
Recent studies suggest that the mammary cytoplasmic estrogen receptor of virgin mice sediment predominantly as 45 species on low ionic strength sucrose gradients. In the present studies we show that the mammary cytoplasmic estrogen receptor from both the intact and ovariectomized virgin and lactating mice sediment at 9S on sucrose gradients prepared in low ionic strength buffers. However, the receptor from the virgin mice is susceptible to cleavage, resulting in the appearance of 3-4S forms of the receptor. If, however, phosphate buffers or buffers containing sodium molybdate are used in the preparation of cytosol and its analysis, it preserves the integrity of the native 9S estrogen receptor.