Conserved expression of the PRELI domain containing 2 gene (Prelid2) during mid-later-gestation mouse embryogenesis
Conserved expression of the PRELI domain containing 2 gene (Prelid2) during mid-later-gestation mouse embryogenesis
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DOI：
10.1007/s10735-009-9234-1
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发表时间：
2009-06-01
影响因子：
3.2
通讯作者：
Wu, Qiong
中科院分区：
文献类型：
--
作者：
Gao, Mengya;Liu, Qi;Wu, Qiong
Preld2属于含有Preli结构域的家族，是一个保守的进化基因。Prelid2基因在胚胎发育过程中的表达和调控尚不清楚。在本研究中，我们利用小鼠胚胎模型，通过原位杂交分析、RT-PCR和亚硫酸氢盐测序，研究了prelid2基因的表达和调控。原位杂交分析表明，Prelid2基因在E9.5和E10.5胚胎的中脑、脊髓、视隆起、耳囊和尾巴中表达，在E13.5和E15.5胚胎的前脑、后脑、心、肺、肝和肾脏中表达。实时定量RT-PCR结果验证了小鼠脑、心、肺、肝四个主要器官在器官分化形成过程中的表达模式。亚硫酸氢盐测序结果表明，在E12.5、E18.5和新生儿的基因组启动子区域表达和去甲基化状态一致。因此，prelid2基因是一个在小鼠胚胎发育中广泛分布、持续表达和未甲基化的基因。我们的结果表明，含有2基因的Preli结构域参与了小鼠胚胎的发育。
Prelid2, which belongs to the PRELI domain containing family, is identified as a conserved evolution gene. The expression and regulation during embryonic development of the prelid2 gene is unknown. In this study, we investigated the prelid2 gene expression and regulation using mouse embryos model, by in situ hybridization analysis, RT-PCR and bisulfite sequencing. In situ hybridization analysis showed that prelid2 gene expression were found in midbrain, spinal cord, optic eminence, otic vesicle and tail at E9.5 and E10.5 embryos, in forebrain, hindbrain, heart, lung, liver and kidney at E13.5 and E15.5 embryos. Real-time quantitative RT-PCR results verified the expression pattern in the four major mouse organs, brain, heart, lung, and liver during organs differentiation and formation. Bisulfite sequencing illustrated the consistent result of expression and its unmethylation status in the genomic promoter region at E12.5, E18.5, and new born. Thus, the prelid2 gene is a widely-spread, persistently expressed and unmethylated gene in mouse embryonic development. Our results suggest that the PRELI domain containing 2 gene is involved in mouse embryonic development.