From Alpha to Beta - a co-translational way to fold?
From Alpha to Beta - a co-translational way to fold?
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DOI：
10.1080/15384101.2022.2062186
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发表时间：
2022-08
期刊：
影响因子：
--
通讯作者：
Komar AA
中科院分区：
文献类型：
--
作者：
Komar AA
细胞中的蛋白质折叠主要是发生在核糖体上的蛋白质合成过程中的共翻译过程。很明显，在原核和真核起源的细胞中，无论是单结构域还是多结构域，单亚基还是多亚基，细胞质、分泌和膜，几乎每一种蛋白质都具有共翻译折叠的特征。在核糖体多肽链合成过程中，共翻译蛋白折叠很早就开始了，一些二级结构元件在核糖体通道内形成，一些三级结构元件在核糖体出口通道的前庭（下/宽）区域形成。然而，共平移折叠的许多细节仍未完全了解。新的数据表明，β-桶状蛋白的折叠始于核糖体内部α-螺旋的形成，当生长的肽到达出口通道的更宽/前厅区域时，α-螺旋结构重新排列成β-发夹结构。虽然以前有人认为这种情况可能发生在核糖体上，但新的数据提供了支持这一观点的第一个实验证据。
Protein folding in the cell is largely a co-translational process occurring during protein synthesis on the ribosome. It has become evident that co-translational folding is characteristic to almost every protein in the cell of pro- and eukaryotic origin that are single and multidomain, single and multisubunit, cytosolic, secretory and membrane. Co-translational protein folding begins very early during the process of polypeptide chain synthesis on the ribosome, with some secondary structure elements forming inside the ribosomal tunnel and some tertiary structures forming inside the vestibule (lower/wider) region of the ribosomal exit tunnel. However, many details of co-translational folding remains incompletely understood. New data show that folding of a β-barrel protein begins with formation of an α-helix inside the ribosome that rearranges into a β-hairpin structure as the growing peptide reaches the wider/vestibule region of the exit tunnel. While it was previously suggested that such scenario can take place on the ribosome, the new data provide the first experimental evidence in support of this notion.
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DOI：
10.15252/embj.2021109175
发表时间：
2022-02-15
期刊：
The EMBO journal
影响因子：
--
作者：
Agirrezabala X;Samatova E;Macher M;Liutkute M;Maiti M;Gil-Carton D;Novacek J;Valle M;Rodnina MV
通讯作者：
Rodnina MV
影响因子：
16.8
作者：
Bhushan, Shashi;Gartmann, Marco;Beckmann, Roland
通讯作者：
Beckmann, Roland
影响因子：
8
作者：
Vu, DM;Reid, KL;Gregoret, LM
通讯作者：
Gregoret, LM
影响因子：
5.6
作者：
LIM, VI;SPIRIN, AS
通讯作者：
SPIRIN, AS
影响因子：
64.5
作者：
Woolhead, CA;McCormick, PJ;Johnson, AE
通讯作者：
Johnson, AE