Analysis of In Vivo Cell Movement Using Transparent Tissue Systems
Analysis of In Vivo Cell Movement Using Transparent Tissue Systems
复制标题
使用透明组织系统分析体内细胞运动
DOI：
10.1242/jcs.1987.supplement_8.22
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发表时间：
1987
影响因子：
4
通讯作者：
A. Wood
中科院分区：
文献类型：
--
作者：
P. Thorogood;A. Wood
某些硬骨鱼物种的胚胎是透明的，使用Nomarski显微镜结合延时录像或延时电影拍摄可以观察和记录完整胚胎内的细胞行为。在这篇报告中，我们回顾了我们最近对细胞行为模式的一些分析，这些模式是关键形态发生事件的基础。(1)鳍发育过程中，接触引导细胞通过结构有序的细胞外基质迁移；(2)表代谢过度生长过程中组织层的运动；(3)在身体轴建立过程中的细胞“社会”行为（即脊索形成和体细胞发生）。这些关于细胞行为与正常形态发生相关的结果，为进一步的工作提供了基础，在这些工作中，有关细胞行为的亚细胞和分子控制的假设可以通过体内实验扰动进行测试。
SUMMARY The embryos of certain teleost species are transparent and cell behaviour within the intact embryo can be observed and recorded using Nomarski microscopy coupled with time-lapse video recording or time-lapse cine filming. In this report we review some of our recent analyses of cell behaviour patterns underlying key morphogenetic events. (1) Contact-guided cell migration through a structurally ordered extracellular matrix during fin development; (2) movement of tissue layers during epibolic overgrowth; and (3) cell ‘social’ behaviour during the establishment of the body axis (i.e. notochord formation and somitogenesis). These results, on cell behaviour correlated with normal morphogenesis, provide a baseline for further work in which hypotheses concerning subcellular and molecular controls of cell behaviour can be tested by experimental perturbation in vivo.