CHARACTERIZATION OF FUNCTIONAL-ORGANIZATION WITHIN RAT BARREL CORTEX USING INTRINSIC SIGNAL OPTICAL IMAGING THROUGH A THINNED SKULL
CHARACTERIZATION OF FUNCTIONAL-ORGANIZATION WITHIN RAT BARREL CORTEX USING INTRINSIC SIGNAL OPTICAL IMAGING THROUGH A THINNED SKULL
复制标题
DOI：
10.1073/pnas.90.21.9998
复制
发表时间：
1993-11-01
影响因子：
11.1
通讯作者：
FROSTIG, RD
中科院分区：
文献类型：
--
作者：
MASINO, SA;KWON, MC;FROSTIG, RD
本研究利用光学成像技术对大鼠体感皮层中神秘触须的功能表征进行了研究。在5hz下刺激单个胡须2秒会导致皮层中一个离散的功能活动区域。通过硬脑膜和变薄的头骨收集胡须的图像。我们用二维图像和三维表面图表征了须在空间和时间上的功能表征，这些图像是皮层响应须刺激的内在信号发展的。单单元记录证实，光学成像获得的须的表征与皮层中该须的电生理反应区域相对应。功能活动中心的病变位于相应须的密集细胞色素氧化酶斑块的中心。此外，对一个3 × 3的晶须矩阵进行了刺激，发现图像表示中心之间的距离与9个晶须第四层细胞色素氧化酶染色中心之间的距离高度相关（r = 0.98）。本研究显示了大鼠体感觉皮层的成像、生理和解剖学之间惊人的对应关系。此外，通过薄颅骨使用光学成像的能力应该可以研究单个动物的感觉表征的长期变化。
We used optical imaging of intrinsic signals to characterize the functional representations of mystacial vibrissae (whiskers) in rat somatosensory cortex. Stimulation of individual whiskers for 2 s at 5 Hz resulted in a discrete area of functional activity in the cortex. Images of whisker representations were collected both through the dura and through a thinned skull. We characterized the functional representation of a whisker both spatially and temporally with two-dimensional images and three-dimensional surface plots of intrinsic signal development in the cortex in response to whisker stimulation. Single unit recordings verified that the representation of the whisker obtained with optical imaging corresponded with the electrophysiological response area of that whisker in the cortex. Lesions in the center of the functional activity were found to be in the center of the dense cytochrome oxidase patch for the corresponding whisker. In addition, a 3 x 3 matrix of whiskers was stimulated and the distances between the centers of the imaged representations and the distances between the centers of the layer IV cytochrome oxidase staining of the nine whiskers were found to be highly correlated (r = 0.98). This study shows a striking correspondence among imaging, physiology, and anatomy in the rat somatosensory cortex. Furthermore, the ability to use optical imaging through a thinned skull should allow investigations into the long-term changes in a sensory representation within a single animal.