Retroviral lineage studies of the rat olfactory epithelium.
Retroviral lineage studies of the rat olfactory epithelium.
复制标题
大鼠嗅上皮的逆转录病毒谱系研究。
DOI：
10.1093/chemse/19.6.671
复制
发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Youngentob,SL
中科院分区：
文献类型：
--
作者：
Schwob,JE;Huard,JM;Luskin,MB;Youngentob,SL
利用编码可遗传标记酶β-半乳糖苷酶的复制能力逆转录病毒载体，研究了未处理动物嗅上皮细胞和损伤后重建嗅上皮细胞的谱系关系。病毒标记的细胞根据其在上皮中的位置、NCAM的表达（仅限于神经元）和griffonialectin识别的碳水化合物部分（仅限于暗/水平基底细胞和微绒毛类支持细胞）进行分类。感染后6-10天，将载体直接注射到其他完好动物的嗅上皮中，可产生β-半乳糖苷酶标记的细胞簇；这些细胞簇完全由神经元和NCAM-阴性/凝集素阴性的光/球状基底细胞组成。相反，mebr诱导的上皮病变后的病毒标记细胞簇通常包含神经元和支持细胞，以及两种类型的基底细胞。其他细胞簇含有支持细胞和/或鲍曼腺/导管细胞。有标记的细胞群很可能来源于一个单一的创始细胞，即细胞是克隆的和直系相关的，因为这些细胞群分布广泛。此外，可以根据所表达的标记酶的死亡类型和亚细胞定位来区分的病毒混合物的输注产生与酶类型相同的簇，这为支持簇本质上是克隆的概念提供了强有力的证据。因此，包含神经元、支持细胞和基底细胞的克隆的创建者在分化能力上是多能的。多能细胞不太可能在鲍曼氏腺/管中发现，因为我们还没有观察到在鲍曼氏腺/管中含有神经元和细胞的克隆。因此，多能干细胞存在于上皮基底细胞区。然而，这些干细胞的确切性质仍然是未知的，也是未来研究的主题。
Replicalion-iocompetent retroviral vecton that encode the heritable marker enzyme, β-galactosidase, were used to study the lineage relationships of cells in the olfactory epithelium of unmanipulated animals and in the olfactory epithelium as it reconstitutes after lesion. Viralry-marked cells are categorized as to type based on their position in the epithelium and on expression of NCAM (limited to neurons) and the carbohydrate moiety recognized byGriffonialectin (limited to the dark/horizontal basal cells and the microvillar class of supporting cells). Direct injections of the vectors into the olfactory epithelium of otherwise intact animals produce clusters of β-galactosidase-labeled cells when assessed 6–10 days after infection; these clusters are composed of neurons and NCAM- negative/lectin-negative light/globose basal cells exclusively. In contrast, clusters of virally-marked cells after MeBr-induced lesion of the epithelium frequently contain both neurons and supporting cells, M well as both types of basal cells. Other clusters contain supporting cells and/or Bowman's gland/duct cells. It is likely that the clusters of marked cells are derived from a single founder cell, i.e. the cells are clonal and lineally related, since the clusters are widely dispersed. Furthermore, infusion of mixtures of viruses that can be distinguished on the basis of die type and subcellular localization of the marker enzyme that is expressed produce clusters that are homogenous with respect to enzyme type, providing strong evidence in favor of the notion that the clusters are clonal in nature. Thus, the founders of the clones that contain neurons, supporting cells and basal cells are pluripotent in their capacity for differentiation. It is unlikely that the pluripotent cells are found in Bowman's gland/duct, since we have yet to observe a clone that contains neurons and cells in Bowman's gland/duct. Hence, the pluripotent stem cells are to be found in die basal cell compartment of the epithelium. However, the exact nature of these stem cells remains unknown and a subject for future investigation.